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Detection of aluminium tolerance plasmids and microbial diversity in the rhizosphere of plants grown in acidic volcanic soil

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dc.contributor.authorJorquera, M.
dc.contributor.authorHernandez, M.
dc.contributor.authorMartinez, O.
dc.contributor.authorMarschner, P.
dc.contributor.authorde la Luz Mora, M.
dc.date.issued2010
dc.description.abstractThe rhizosphere is considered as a hot-spot for gene exchange among bacteria in terrestrial ecosystems. Chilean volcanic soils are characterized by low pH and high concentrations of aluminium (Al) in the soil solution, thus Al tolerance could be important for the survival of microorganisms in these soils; loss of genes encoding for Al tolerance may affect competitiveness particularly in the rhizosphere where competition is strong. The occurrence of Al-tolerance plasmids was investigated in the rhizospheres of pasture and crop plants growing in acidic volcanic soils from southern Chile. Al tolerance plasmids were captured by biparental mating. Two types of Al tolerance plasmids could be distinguished, based on their endonuclease restriction pattern. One plasmid of each group (denoted as pRPA21 and pOPA21) was selected for further studies. The plasmids showed a high stability in presence and absence of Al. Additionally, microbial community composition in the rhizosphere soils was assessed by denaturing gradient gel electrophoresis (DGGE). Sequencing of DGGE bands revealed among others, members of the bacterial phylum Gemmatimonadetes and archaeal phylum Crenarchaeota. The present study shows that the rhizosphere of pasture and crop plants growing in Chilean volcanic soil harbors genetic mobile elements which could play a role in the adaptation of bacterial populations to environmental stressors, such as Al-toxicity. © 2010 Elsevier Masson SAS.
dc.description.statementofresponsibilityMilko A. Jorquera, Marcela Hernández, Oscar Martínez, Petra Marschner, Maríadela Luz Mora
dc.identifier.citationEuropean Journal of Soil Biology, 2010; 46(3-4):255-263
dc.identifier.doi10.1016/j.ejsobi.2010.03.005
dc.identifier.issn1164-5563
dc.identifier.issn1778-3615
dc.identifier.orcidMarschner, P. [0000-0001-6808-0244]
dc.identifier.urihttp://hdl.handle.net/2440/61339
dc.language.isoen
dc.publisherGauthier-Villars/Editions Elsevier
dc.rightsCopyright © 2010 Elsevier Masson SAS. All rights reserved.
dc.source.urihttps://doi.org/10.1016/j.ejsobi.2010.03.005
dc.subjectAcidic soils
dc.subjectAluminium tolerance
dc.subjectMicrobial community
dc.subjectPlasmid
dc.subjectRhizosphere
dc.titleDetection of aluminium tolerance plasmids and microbial diversity in the rhizosphere of plants grown in acidic volcanic soil
dc.typeJournal article
pubs.publication-statusPublished

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