Effects of Streptococcus thermophilus TH-4 on intestinal mucositis induced by the chemotherapeutic agent 5-Fluorouracil (5-FU)
Date
2009
Authors
Whitford, E.
Cummins, A.
Butler, R.
Prisciandaro, L.
Fauser, J.
Yazbek, R.
Lawrence, A.
Cheah, K.
Wright, T.
Lymn, K.
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Journal article
Citation
Cancer Biology & Therapy, 2009; 8(6):505-511
Statement of Responsibility
E.J. Whitford, A.G. Cummins, R.N. Butler, L.D. Prisciandaro, J.K. Fauser, R. Yazbeck, A. Lawrence, K.Y. Cheah, T.H. Wright, Kerry Lymn and G.S. Howarth
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Abstract
Beneficial bacteria (probiotics) and probiotic-derived factors have the potential to ameliorate disorders of the intestine. The aim of this study was to compare live Streptococcus thermophilus TH-4 (TH-4), dead TH-4 and TH-4 supernatant in rats treated with 5-Fluorouracil. Rats were randomly allocated to five treatment groups (n=8-10): Saline+Water; 5-FU+Skim Milk; 5-FU+Live TH-4; 5-FU+Supernatant TH-4; and 5-FU+Dead TH-4. 5-FU (150mg.kg-1) was administered by a single intraperitoneal injection on day 0; animals were killed on day 4. Treatments were administered daily from days -2 to 3 via oro-gastric gavage. Metabolic parameters were measured daily. Blood was obtained by cardiac puncture, and intestinal tissues removed for quantitative and qualitative histological assessment, including: villous height and area; crypt depth and area, mitotic count and crypt fission; biochemical determination of sucrase and myeloperoxidase (MPO) activity; and disease severity scoring. One-way ANOVA statistical analyses were conducted for the majority of outcome measures. Live TH-4 significantly reduced disease severity score by 13% (p
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© 2009 Landes Bioscience