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Effect of lipid and fatty acid composition of phospholipid vesicles on long-term stability and their response to Staphylococcus aureus and Pseudomonas aeruginosa supernatants

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dc.contributor.authorMarshall, S.E.
dc.contributor.authorHong, S.H.
dc.contributor.authorThet, N.T.
dc.contributor.authorJenkins, A.T.A.
dc.date.issued2013
dc.descriptionData source: Supporting information, https://doi.org/10.1021/la401679u
dc.description.abstractPhospholipid vesicles have been the focus of attention as potential vehicles for drug delivery, as they are biomimetic, easy to produce, and contain an aqueous compartment which can be used to carry hydrophilic material, such as drugs or dyes. Lipid vesicles used for this purpose present a particular challenge, as they are not especially stable and can rapidly break down and release their contents away from the target area, especially at physiological temperatures/environments. This study aims to investigate optimum methods for vesicle stabilization where the vesicles are employed as part of a system or technology that signals the presence of pathogenic bacteria via the effect of secreted cytolytic virulence factors on a sensor interface.(1) A number of approaches have been investigated and are presented here as a systematic study of the long-term (14 day) stability at 37 C, and at various pHs. The response of vesicles, both in suspension and within hydrogels, to Staphylococcus aureus (RN 4282) and Pseudomonas aeruginosa (PAO1) whole bacteria, and supernatants from overnight cultures of both (containing secreted proteins but free of cells), was measured via a sensitive encapsulated carboxyfluorescein release assay. The results showed that lipid chain length, cholesterol concentration, and stabilization via photopolymer stable components were critical in achieving stability. Finally, dispersion of the optimum vesicle formulation in hydrogel matrixes was investigated, culminating in the in vivo demonstration of a simple prototype wound dressing.
dc.identifier.citationLangmuir, 2013; 29(23):6989-6995
dc.identifier.doi10.1021/la401679u
dc.identifier.issn0743-7463
dc.identifier.issn1520-5827
dc.identifier.urihttps://hdl.handle.net/11541.2/147671
dc.language.isoen
dc.publisherAmerican Chemical Society
dc.relation.fundingEPSRC (CSCT)
dc.relation.fundingEC 7th Framework Bacteriosafe 245500
dc.rightsCopyright 2013 American Chemical Society
dc.source.urihttps://doi.org/10.1021/la401679u
dc.subjectfatty acid
dc.subjectlipid
dc.subjectPseudomonas aeruginosa
dc.subjectStaphylococcus aureus
dc.subjectpathogenic bacterium
dc.subjectphospholipid vesicles
dc.subjectliposomes
dc.titleEffect of lipid and fatty acid composition of phospholipid vesicles on long-term stability and their response to Staphylococcus aureus and Pseudomonas aeruginosa supernatants
dc.typeJournal article
pubs.publication-statusPublished
ror.mmsid9916530200501831

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