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Immortalized mouse caput epididymal epithelial (mECap18) cell line recapitulates the in-vivo environment

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dc.contributor.authorMulhall, J.E.
dc.contributor.authorTrigg, N.A.
dc.contributor.authorBernstein, I.R.
dc.contributor.authorAnderson, A.L.
dc.contributor.authorMurray, H.C.
dc.contributor.authorSipilä, P.
dc.contributor.authorLord, T.
dc.contributor.authorSchjenken, J.E.
dc.contributor.authorNixon, B.
dc.contributor.authorSkerrett-Byrne, D.A.
dc.date.issued2024
dc.descriptionFirst published: 27 September 2023
dc.description.abstractResiding between the testes and the vas deferens, the epididymis is a highly convoluted tubule whose unique luminal microenvironment is crucial for the functional maturation of spermatozoa. This microenvironment is created by the combined secretory and resorptive activity of the lining epididymal epithelium, including the release of extracellular vesicles (epididymosomes), which encapsulate fertility modulating proteins and a myriad of small non-coding RNAs (sncRNAs) that are destined for delivery to recipient sperm cells. To enable investigation of this intercellular communication nexus, we have previously developed an immortalized mouse caput epididymal epithelial cell line (mECap18). Here, we describe the application of label-free mass spectrometry to characterize the mECap18 cell proteome and compare this to the proteome of native mouse caput epididymal epithelial cells. We report the identification of 5,313 mECap18 proteins, as many as 75.8% of which were also identified in caput epithelial cells wherein they mapped to broadly similar protein classification groupings. Furthermore, key pathways associated with protein synthesis (e.g., EIF2 signaling) and cellular protection in the male reproductive tract (e.g., sirtuin signaling) were enriched in both proteomes. This comparison supports the utility of the mECap18 cell line as a tractable in-vitro model for studying caput epididymal epithelial cell function.
dc.description.statementofresponsibilityJess E. Mulhall, Natalie A. Trigg, Ilana R. Bernstein, Amanda L. Anderson, Heather C. Murray, Petra Sipilä, Tessa Lord, John E. Schjenken, Brett Nixon, David A. Skerrett-Byrne
dc.identifier.citationProteomics, 2024; 24(7):2300253-1-2300253-9
dc.identifier.doi10.1002/pmic.202300253
dc.identifier.issn1615-9853
dc.identifier.issn1615-9861
dc.identifier.orcidSchjenken, J.E. [0000-0001-6293-6160]
dc.identifier.urihttps://hdl.handle.net/2440/139800
dc.language.isoen
dc.publisherWiley
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/1147932
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/1154837
dc.rights© 2023 The Authors. Proteomics published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
dc.source.urihttps://doi.org/10.1002/pmic.202300253
dc.subjectepididymis
dc.subjectfertility
dc.subjectmale reproduction
dc.subjectproteomics
dc.subjectsperm maturation
dc.titleImmortalized mouse caput epididymal epithelial (mECap18) cell line recapitulates the in-vivo environment
dc.typeJournal article
pubs.publication-statusPublished

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