Mapping of chromosomal loci associated with lipopolysaccharide synthesis and serotype specificity in Vibrio cholerae 01 by transposon mutagenesis using Tn5 and Tn2680

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dc.contributor.authorWard, H.
dc.contributor.authorManning, P.
dc.date.issued1989
dc.description.abstractVibrio cholerae strains of the 01 serotype have been classified into three subclasses, Ogawa, Inaba and Hikojima, which are associated with the O-antigen of the lipopolysaccharide (LPS). The DNA encoding the biosynthesis of the O-antigen, the rfb locus, has been cloned and analysed (Manning et al. 1986; Ward et al. 1987). Transposon mutagenesis of the Inaba and Ogawa strains of V. cholerae, using Tn5 or Tn2680 allowed the isolation of a series of independent mutants in each of these serotypes. Some of the insertions were mapped to the rfb region by Southern hybridization using the cloned rfb DNA as a probe, confirming this location to be responsible for both O-antigen production and serotype specificity. The other insertions allowed a second region to be identified which is involved in V. cholerae LPS biosynthesis.
dc.description.statementofresponsibilityHelena M. Ward and Paul A. Manning
dc.identifier.citationMolecular Genetics and Genomics: an international journal, 1989; 218(2):367-370
dc.identifier.doi10.1007/BF00331294
dc.identifier.issn0026-8925
dc.identifier.issn1432-1874
dc.identifier.orcidWard, H. [0000-0002-3831-1205]
dc.identifier.urihttp://hdl.handle.net/2440/81806
dc.language.isoen
dc.publisherSpringer Verlag
dc.rights© Springer-Verlag 1989
dc.source.urihttps://doi.org/10.1007/bf00331294
dc.subjectBacteriophage resistance, O-antigen
dc.subjectConjugation
dc.subjectLPS
dc.subjectP-factor
dc.titleMapping of chromosomal loci associated with lipopolysaccharide synthesis and serotype specificity in Vibrio cholerae 01 by transposon mutagenesis using Tn5 and Tn2680
dc.typeJournal article
pubs.publication-statusPublished

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