A combined free-flow electrophoresis and DIGE approach to identify proteins regulated by butyrate in HT29 cells

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dc.contributor.authorFung, K.
dc.contributor.authorCursaro, V.
dc.contributor.authorLewanowitsch, T.
dc.contributor.authorBrierley, G.
dc.contributor.authorMcColl, S.
dc.contributor.authorLockett, T.
dc.contributor.authorHead, R.
dc.contributor.authorHoffmann, P.
dc.contributor.authorCosgrove, L.
dc.date.issued2011
dc.descriptionArticle first published online: 31 JAN 2011
dc.description.abstractMany biologically active agents exert a pleiotropic response in cells and tissues. This presents challenges in descriptive and comparative analysis of the proteome in response to these agents. Although free-flow electrophoresis has been applied in a number of proteomic studies as a protein separation technique, the combination of free-flow electrophoresis and DIGE has not yet been investigated for comparative proteomic analysis. In this study, we have compared the effects of butyrate on HT29 colorectal cancer cells with a particular focus on apoptosis and describe the utility of a novel approach combining free-flow electrophoresis with DIGE to identify differentially expressed proteins. We verify the results obtained by the combined free-flow electrophoresis and DIGE approach with Western blot analysis of selected proteins. We also report for the first time the regulation of a number of proteins by butyrate in HT29 colorectal cells including peptidyl-prolyl cis-trans isomerase A (cyclophilin A) and profilin-1.
dc.description.statementofresponsibilityKim Y. C. Fung, Chris Cursaro, Tanya Lewanowitsch, Gemma V. Brierley, Shaun R. McColl, Trevor Lockett, Richard Head, Peter Hoffmann and Leah Cosgrove
dc.identifier.citationProteomics, 2011; 11(5):964-971
dc.identifier.doi10.1002/pmic.201000429
dc.identifier.issn1615-9853
dc.identifier.issn1615-9861
dc.identifier.orcidMcColl, S. [0000-0003-0949-4660]
dc.identifier.orcidHead, R. [0000-0002-1196-0926]
dc.identifier.urihttp://hdl.handle.net/2440/66076
dc.language.isoen
dc.publisherWiley - V C H Verlag GmbH & Co. KGaA
dc.rightsCopyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
dc.source.urihttps://doi.org/10.1002/pmic.201000429
dc.subjectButyrate
dc.subjectColorectal cancer
dc.subjectDIGE
dc.subjectFree-flow electrophoresis
dc.subjectTechnology
dc.titleA combined free-flow electrophoresis and DIGE approach to identify proteins regulated by butyrate in HT29 cells
dc.typeJournal article
pubs.publication-statusPublished

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