The grapes checkpoint coordinates nuclear envelope breakdown and chromosome condensation

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dc.contributor.authorYu, K.
dc.contributor.authorSaint, R.
dc.contributor.authorSullivan, W.
dc.contributor.organisationCentre for the Molecular Genetics of Development
dc.date.issued2000
dc.description.abstractMutations in the embryonic Drosophila Grapes/Chk1 checkpoint result in an abbreviated interphase, chromosome condensation defects and metaphase delays. To clarify the relationship between these phenotypes, we simultaneously timed multiple nuclear and cytoplasmic events in mutant grp-derived embryos. These studies support a model in which grp disrupts an S-phase checkpoint, which results in progression into metaphase with incompletely replicated chromosomes. We also show that chromosome condensation is independent of the state of DNA replication in the early embryo. Therefore, grp condensation defects are not a direct consequence of entering metaphase with incompletely replicated chromosomes. Rather, initiation of chromosome condensation (ICC) occurs at the normal time in grp-derived embryos, but the shortened interval between ICC and metaphase does not provide sufficient time to complete condensation. Our results suggest that these condensation defects, rather than incomplete DNA replication, are responsible for the extensive metaphase delays observed in grp-derived embryos. This analysis provides an example of how the loss of a checkpoint can disrupt the timing of multiple events not directly monitored by that checkpoint. These results are likely to apply to vertebrate cells and suggest new strategies for destroying checkpoint-compromised cancer cells.
dc.identifier.citationNature Cell Biology, 2000; 2(9):609-615
dc.identifier.doi10.1038/35023555
dc.identifier.issn1465-7392
dc.identifier.issn1476-4679
dc.identifier.urihttp://hdl.handle.net/2440/28003
dc.language.isoen
dc.publisherNature Publishing Group
dc.source.urihttp://www.nature.com/ncb/journal/v2/n9/abs/ncb0900_609.html
dc.subjectNuclear Envelope
dc.subjectCell Nucleus
dc.subjectAnimals
dc.subjectDrosophila
dc.subjectProtein Kinases
dc.subjectDrosophila Proteins
dc.subjectSignal Transduction
dc.subjectChromosome Segregation
dc.subjectMetaphase
dc.subjectDNA Replication
dc.subjectGenes, Insect
dc.subjectTime Factors
dc.subjectCheckpoint Kinase 1
dc.titleThe grapes checkpoint coordinates nuclear envelope breakdown and chromosome condensation
dc.typeJournal article
pubs.publication-statusPublished

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