Promoters of cancer genes for recombinant protein expression in human cancer cell lines
Date
2012
Authors
Pourhassan Moghaddam, M.
Farhadi, B.
Nejati Koshki, K.
Moharrami, T.
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Journal article
Citation
BioImpacts, 2012; 2(1):55-60
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Abstract
Introduction: Production of complex human recombinant proteins is an important issue in medical biotechnology. These proteins are mostly expressed in non-human mammalian host cells. This has some problems including non-human post-translational modifications, application of high-cost agents for inducing protein expression and low yields. Thus, it is necessary to use new expression systems to overcome the indicated challenges.
Methods: In this paper, we hypothesize the application of promoter regions of cancer genes, which have a high rate of transcription in human cancer cell lines, for designing new expression vectors.
Results: After designing, these vectors could be applied to produce complex human recombinant proteins in the human cancer cell lines as production hosts.
Conclusion: Application of these expression vectors for the production of recombinant human proteins in the human cancer cell lines have some advantages including authentic post-translational modifications, proper-cost of commercialization, and high yields.
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Copyright 2012 The Author(s). This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (https://creativecommons.org/licenses/by-nc/4.0/)