Isolation and characterization of microsatellite markers for the Litoria ewingii complex and their use in conservation and hybridization studies

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dc.contributor.authorSmith, K.
dc.contributor.authorHale, J.
dc.contributor.authorAustin, J.
dc.contributor.authorMelville, J.
dc.date.issued2011
dc.description.abstractEight polymorphic microsatellite loci were identified in the Australian tree frog, Litoria ewingii, for studying patterns of hybridization with the closely related species L. paraewingi, using a microsatellite enriched genomic library. Across 55 individuals of L. ewingii and L. paraewingi, the mean number of alleles per locus was Na=9.38 and Na=7.13, respectively. All loci successfully cross-amplified in the remaining species in the Litoria ewingii complex, including the threatened sub-species L. verreauxii alpina. These loci will be useful in resolving species boundaries and exploring hybridization between species in the Litoria ewingii complex.
dc.description.statementofresponsibilityKatie L. Smith, Joshua M. Hale, Jeremy J. Austin, Jane Melville
dc.identifier.citationConservation Genetics Resources, 2011; 3(4):621-624
dc.identifier.doi10.1007/s12686-011-9418-3
dc.identifier.issn1877-7252
dc.identifier.issn1877-7260
dc.identifier.orcidAustin, J. [0000-0003-4244-2942]
dc.identifier.urihttp://hdl.handle.net/2440/67160
dc.language.isoen
dc.publisherSpringer Netherlands
dc.relation.grantARC
dc.rights© Springer Science+Business Media B.V. 2011
dc.source.urihttps://doi.org/10.1007/s12686-011-9418-3
dc.subjectHybrid zone
dc.subjectMicrosatellite
dc.subjectLitoria ewingii
dc.subjectLitoria paraewingi
dc.subjectLitoria verreauxii alpina
dc.subjectAmphibian
dc.subjectSouth-eastern Australia
dc.subjectTree frog
dc.subjectSpecies boundaries
dc.subjectHybridization
dc.titleIsolation and characterization of microsatellite markers for the Litoria ewingii complex and their use in conservation and hybridization studies
dc.typeJournal article
pubs.publication-statusPublished

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