Chimeric caspase molecules with potent cell killing activity in apoptosis-resistant cells

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dc.contributor.authorShearwin-Whyatt, L.
dc.contributor.authorBaliga, B.
dc.contributor.authorDoumanis, J.
dc.contributor.authorKumar, S.
dc.date.issued2001
dc.descriptionCopyright © 2001 Academic Press. All rights reserved.
dc.description.abstractCellular defects which prevent apoptotic cell death can result in the generation of hyperproliferative disorders and can prevent the effective treatment of such diseases. The majority of cellular defects which result in apoptosis resistance lie upstream of caspase activation. We have described chimeric caspase molecules consisting of the prodomain of caspase-2 fused to the amino terminus of caspase-3, and which are tagged at the carboxyl terminus with green fluorescent protein (GFP) to allow direct visualisation of transfected cells. Here we show that these chimeric caspase molecules possess potent, rapid cell-killing activity in cell lines which display a range of defects resulting in apoptosis resistance.
dc.description.statementofresponsibilityLinda Shearwin-Whyatt, Belinda Baliga, Joanna Doumanis and Sharad Kumar
dc.description.urihttp://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description#description
dc.identifier.citationBiochemical and Biophysical Research Communications, 2001; 282(5):1114-1119
dc.identifier.doi10.1006/bbrc.2001.4699
dc.identifier.issn0006-291X
dc.identifier.issn1090-2104
dc.identifier.orcidShearwin-Whyatt, L. [0000-0002-4504-6534]
dc.identifier.orcidKumar, S. [0000-0001-7126-9814]
dc.identifier.urihttp://hdl.handle.net/2440/9291
dc.language.isoen
dc.publisherAcademic Press Inc
dc.source.urihttps://doi.org/10.1006/bbrc.2001.4699
dc.subjectCOS Cells
dc.subjectTumor Cells, Cultured
dc.subjectAnimals
dc.subjectHumans
dc.subjectLeukemia, T-Cell
dc.subjectOsteosarcoma
dc.subjectCaspases
dc.subjectLuminescent Proteins
dc.subjectGreen Fluorescent Proteins
dc.subjectRecombinant Fusion Proteins
dc.subjectTransfection
dc.subjectDrug Resistance, Multiple
dc.subjectApoptosis
dc.subjectCell Survival
dc.subjectDrug Resistance, Neoplasm
dc.subjectCaspase 3
dc.subjectCaspase 2
dc.subjectLeukemia, Myelogenous, Chronic, BCR-ABL Positive
dc.subjectATP Binding Cassette Transporter, Subfamily B, Member 1
dc.titleChimeric caspase molecules with potent cell killing activity in apoptosis-resistant cells
dc.typeJournal article
pubs.publication-statusPublished

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