A new biocomposite based on microbial autocrine laccase-rice straw: preparation, characterization and application
Date
2022
Authors
Wang, L.
Xue, C.
Owen, G.
Chen, Z.
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Journal article
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Industrial Crops and Products, 2022; 189(115746):1-11
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The immobilization of laccase on crop residues is a promising way to treat water containing bisphenol A (BPA), but the high cost of commercially available solutions of this enzyme indicates a need for research into feasible cost-effective alternatives. Here, a new biocomposite was developed based on a microbial autocrine laccase process, where rice straw served as the sole carbon source and as a carrier mixed with Comamonas testosteroni FJ17 (C. testosteroni FJ17). A laccase-rice straw biocomposite (RS-Lac) was prepared via a one-step synthesis and subsequently evaluated for the removal of BPA. A response surface methodology was used to optimize the synthesis of RS-Lac, where the subsequent removal efficiency of BPA (500 μg L−1) by RS-Lac biocomposite increased from 46.1 (unoptimized) to 57.0% (optimized). The molecular weight (Mw) of laccase was identified by SDS-PAGE to be 34 kDa. SEM, CLSM, FTIR, and XRD results confirmed laccase secreted by C. testosteroni FJ17 was coated with polysaccharides, and these were successfully immobilized on rice straw. The specific surface area of RS-Lac increased from 2.79 to 5.37 m2 g−1 following synthesis optimization, and zeta potential analyzer indicated that it carried a strong negative charge. In addition, RS-Lac was also used to successfully remove estrone (E1), 17β-estradiol (E2), Cu(II), Sb(Ⅲ), As(Ⅲ), and As(Ⅴ) to varying degrees, with removal efficiencies of 68.7%, 66.4%, 77.5%, 84.0%, 83.7%, and 91.6%, respectively. This broad versatility indicated that RS-Lac has great potential as a low-cost biocomposite for treating wastewater.
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Copyright 2022 Elsevier B.V.