A repetitive DNA sequence common to the different B chromosomes of the genus Brachycome
Date
1997
Authors
Houben, A.
Leach, C.
Verlin, D.
Rofe, R.
Timmis, J.
Editors
Advisors
Journal Title
Journal ISSN
Volume Title
Type:
Journal article
Citation
Chromosoma, 1997; 106(8):513-519
Statement of Responsibility
Houben, Andreas ; Leach, Carolyn R. ; Verlin, Dawn ; Rofe, Ruth ; Timmis, Jeremy N.
Conference Name
Abstract
Dot-like micro B chromosomes of Brachycome dichromosomatica were analysed for their sequence composition. Southern hybridization patterns of a total micro B probe to genomic DNA from plants with and without micro Bs demonstrated that the micro Bs shared sequences with the A chromosomes. In addition to telomere, rDNA and common A and B chromosome sequences, a new B-specific, highly methylated tandem repeat (Bdm29) was detected. After in situ hybridization with Bdm29 the entire micro B chromosome was labelled and clustering of the condensed micro Bs could be observed at interphase. A high number of Bdm29-like sequences were also found in the larger B chromosomes of B. dichromosomatica and in other Bs within the genus Brachycome.