Promotion of cell growth and adhesion of a peptide hydrogel scaffold via mTOR/cadherin signaling

Simple item page
dc.contributor.authorWei, G.
dc.contributor.authorWang, L.
dc.contributor.authorDong, D.
dc.contributor.authorTeng, Z.
dc.contributor.authorShi, Z.
dc.contributor.authorWang, K.
dc.contributor.authorAn, G.
dc.contributor.authorGuan, Y.
dc.contributor.authorHan, B.
dc.contributor.authorYao, M.
dc.contributor.authorXian, C.J.
dc.date.issued2018
dc.description.abstractUnderstanding neurite outgrowth, orientation, and migration is important for the design of biomaterials that interface with the neural tissue. However, the molecular signaling alternations have not been well elucidated to explain the impact of hydrogels on cell morphology. In our previous studies, a silk fibroin peptide (SF16) hydrogel was found to be an effective matrix for the viability, morphology, and proliferation of PC12 rat pheocrhomocytoma cells. We found that PC12 cells in the peptide hydrogel exhibited adhesive morphology compared to those cultured in agarose or collagen. Moreover, we identified that cell adhesion molecules (E- and N-cadherin) controlled by mTOR signaling were highly induced in PC12 cells cultured in the SF16 peptide hydrogel. Our findings suggest that the SF16 peptide might be suitable to be a cell-adhesion material in cell culture or tissue engineering, and mTOR/cadherin signaling is required for the cell adhesion in the SF16-peptide hydrogel.
dc.description.statementofresponsibilityGuojun Wei, Liping Wang, Daming Dong, Zhaowei Teng, Zuowei Shi, Kaifu Wang, Gang An, Ying Guan, Bo Han, Meng Yao, Cory J. Xian
dc.identifier.citationJournal of Cellular Physiology, 2018; 233(2):822-829
dc.identifier.doi10.1002/jcp.25864
dc.identifier.issn0021-9541
dc.identifier.issn1097-4652
dc.identifier.orcidXian, C.J. [0000-0002-8467-2845]
dc.identifier.urihttp://hdl.handle.net/2440/117414
dc.language.isoen
dc.publisherWILEY
dc.relation.grant81371368
dc.relation.grant81300785
dc.relation.grant81671928
dc.relation.grantNHMRC
dc.rights© 2017 Wiley Periodicals, Inc.
dc.source.urihttps://doi.org/10.1002/jcp.25864
dc.subjectCadherin; hydrogel; mTOR; PC12 cells; SF16 peptide
dc.titlePromotion of cell growth and adhesion of a peptide hydrogel scaffold via mTOR/cadherin signaling
dc.typeJournal article
pubs.publication-statusPublished

Files

Collections

Zoology publications