The effects of markedly raised intracellular sphingosine kinase-1 activity in endothelial cells

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dc.contributor.authorLimaye, V.
dc.contributor.authorVadas, M.
dc.contributor.authorPitson, S.
dc.contributor.authorGamble, J.
dc.date.issued2009
dc.description.abstractThe enzyme sphingosine kinase-1 (SK1) promotes the formation of sphingosine-1-phosphate (S1P), which is an important survival factor for endothelial cells (EC). Modest increases in intracellular SK1 activity in the EC are known to confer a survival advantage upon the cells. Here, we investigated the effects of more dramatic increases in intracellular SK1 in the EC. We found that these cells show reduced cell survival under conditions of stress, enhanced caspase-3 activity, cell cycle inhibition, and cell-cell junction disruption. We propose that alterations in the phosphorylation state of the enzyme may explain the differential effects on the phenotype with modest versus high levels of enforced expression of SK1. Our results suggest that SK1 activity is subject to control in the EC, and that this control may be lost in conditions involving vascular regression.
dc.description.statementofresponsibilityVidya Limaye, Mathew A. Vadas, Stuart M. Pitson and Jennifer R. Gamble
dc.identifier.citationCellular & Molecular Biology Letters, 2009; 14(3):411-423
dc.identifier.doi10.2478/s11658-009-0008-2
dc.identifier.issn1425-8153
dc.identifier.issn1689-1392
dc.identifier.orcidLimaye, V. [0000-0002-8142-9532]
dc.identifier.orcidPitson, S. [0000-0002-9527-2740]
dc.identifier.urihttp://hdl.handle.net/2440/60627
dc.language.isoen
dc.publisherCellular & Molecular Biology Letters
dc.relation.grantNHMRC
dc.rights© 2009 by the University of Wrocław, Poland
dc.source.urihttps://doi.org/10.2478/s11658-009-0008-2
dc.subjectSphingosine kinase-1
dc.subjectEndothelial cells
dc.subjectCell survival
dc.titleThe effects of markedly raised intracellular sphingosine kinase-1 activity in endothelial cells
dc.typeJournal article
pubs.publication-statusPublished

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