Regional and oyster microenvironmental scale heterogeneity in the Pacific oyster bacterial community.

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dc.contributor.authorKing, W.L.
dc.contributor.authorSiboni, N.
dc.contributor.authorKahlke, T.
dc.contributor.authorDove, M.
dc.contributor.authorO'Connor, W.
dc.contributor.authorMahbub, K.R.
dc.contributor.authorJenkins, C.
dc.contributor.authorSeymour, J.R.
dc.contributor.authorLabbate, M.
dc.date.issued2020
dc.description.abstractDifferent organs of a host represent distinct microenvironments resulting in the establishment of multiple discrete bacterial communities within a host. These discrete bacterial communities can also vary according to geographical location. For the Pacific oyster, Crassostrea gigas, the factors governing bacterial diversity and abundance of different oyster microenvironments are poorly understood. In this study, the factors shaping bacterial abundance, diversity and composition associated with the C. gigas mantle, gill, adductor muscle and digestive gland were characterised using 16S (V3-V4) rRNA amplicon sequencing across six discrete estuaries. Both location and tissue-type, with tissue-type being the stronger determinant, were factors driving bacterial community composition. Bacterial communities from wave-dominated estuaries had similar compositions and higher bacterial abundance despite being geographically distant from one another, possibly indicating that functional estuarine morphology characteristics are a factor shaping the oyster bacterial community. Despite the bacterial community heterogeneity, examinations of the core bacterial community identified Spirochaetaceae bacteria as conserved across all sites and samples. Whereas members of the Vulcaniibacterium, Spirochaetaceae and Margulisbacteria, and Polynucleobacter were regionally conserved members of the digestive gland, gill and mantle bacterial communities, respectively. This indicates that baseline bacterial community profiles for specific locations are necessary when investigating bacterial communities in oyster health.
dc.description.statementofresponsibilityWilliam L King, Nachshon Siboni, Tim Kahlke, Michael Dove, Wayne O'Connor, Khandaker Rayhan Mahbub, Cheryl Jenkins, Justin R Seymour, and Maurizio Labbate
dc.identifier.citationFEMS Microbiology Ecology, 2020; 96(5):fiaa054-fiaa054
dc.identifier.doi10.1093/femsec/fiaa054
dc.identifier.issn0168-6496
dc.identifier.issn1574-6941
dc.identifier.orcidMahbub, K.R. [0000-0001-7086-9965]
dc.identifier.urihttps://hdl.handle.net/2440/139298
dc.language.isoen
dc.publisherOxford University Press (OUP)
dc.relation.granthttp://purl.org/au-research/grants/arc/LP160101785
dc.rights© FEMS 2020. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com
dc.source.urihttps://doi.org/10.1093/femsec/fiaa054
dc.subjectCrassostrea; microbiome; microenvironments; core microbiome; bivalve; invertebrate microbiota
dc.subject.meshGills
dc.subject.meshAnimals
dc.subject.meshBacteria
dc.subject.meshRNA, Ribosomal, 16S
dc.subject.meshCrassostrea
dc.subject.meshMicrobiota
dc.titleRegional and oyster microenvironmental scale heterogeneity in the Pacific oyster bacterial community.
dc.typeJournal article
pubs.publication-statusPublished

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