Antibody-modified iron oxide nanoparticles for efficient magnetic isolation and flow cytometric determination of L-pneumophila

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dc.contributor.authorBloemen, M.
dc.contributor.authorDenis, C.
dc.contributor.authorPeeters, M.
dc.contributor.authorDe Meester, L.
dc.contributor.authorGils, A.
dc.contributor.authorGeukens, N.
dc.contributor.authorVerbiest, T.
dc.date.issued2015
dc.description.abstractWe report on the design of superparamagnetic nanoparticles capable of selectively isolating targeted bacteria (Legionella pneumophila, serogroup 1) from aqueous solutions. The surface of magnetite nanoparticles (NP) was functionalized with a heterobifunctional poly(ethylene glycol) ligand containing reactive groups for covalent coupling of polyclonal antibodies against L. pneumophila. These bioconjugates were used to label and magnetically isolate L. pneumophila. Flow cytometry revealed high separation and efficiency in this regard. The strain specificity and efficiency of the magnetic NP was tested with recombinant strains of E. coli (expressing the red fluorescent protein) and L. pneumophila (expressing the green fluorescent protein). The detection limit of the method (by flow cytometry) is 104 cells∙mL-1. The results indicate that the new multifunctional NPs are capable of selectively attracting pathogens from a complex mixture and with high efficiency. This, conceivably, paves the way to pre-concentration protocols for numerous other pathogens.
dc.description.statementofresponsibilityMaarten Bloemen, Carla Denis, Miet Peeters, Luc De Meester, Ann Gils, Nick Geukens and Thierry Verbiest
dc.identifier.citationMicrochimica Acta: an international journal on micro and trace analysis, 2015; 182(7-8):1439-1446
dc.identifier.doi10.1007/s00604-015-1466-z
dc.identifier.issn0026-3672
dc.identifier.issn1436-5073
dc.identifier.orcidPeeters, M. [0000-0003-4969-2303]
dc.identifier.urihttp://hdl.handle.net/2440/126906
dc.language.isoen
dc.publisherSpringer
dc.rights© 2015, Springer Nature
dc.source.urihttp://dx.doi.org/10.1007/s00604-015-1466-z
dc.subjectImmunomagnetic isolation; iron oxide; nanoparticles; antibodies; superparamagnetism; heterobifunctional PEG; legionella pneumophila
dc.titleAntibody-modified iron oxide nanoparticles for efficient magnetic isolation and flow cytometric determination of L-pneumophila
dc.typeJournal article
pubs.publication-statusPublished

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