OsPAP10c, a novel secreted acid phosphatase in rice, plays an important role in the utilization of external organic phosphorus

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dc.contributor.authorLu, L.
dc.contributor.authorQiu, W.
dc.contributor.authorGao, W.
dc.contributor.authorTyerman, S.
dc.contributor.authorShou, H.
dc.contributor.authorWang, C.
dc.date.issued2016
dc.description.abstractUnder phosphate (Pi ) starvation, plants increase the secretion of purple acid phosphatases (PAPs) into the rhizosphere to scavenge organic phosphorus (P) for plant use. To date, only a few members of the PAP family have been characterized in crops. In this study, we identified a novel secreted PAP in rice, OsPAP10c, and investigated its role in the utilization of external organic P. OsPAP10c belongs to a monocotyledon-specific subclass of Ia group PAPs and is specifically expressed in the epidermis/exodermis cell layers of roots. Both the transcript and protein levels of OsPAP10c are strongly induced by Pi starvation. OsPAP10c overexpression increased acid phosphatase (APase) activity by more than 10-fold in the culture media and almost fivefold in both roots and leaves under Pi -sufficient and Pi -deficient conditions. This increase in APase activity further improved the plant utilization efficiency of external organic P. Moreover, several APase isoforms corresponding to OsPAP10c were identified using in-gel activity assays. Under field conditions with three different Pi supply levels, OsPAP10c-overexpressing plants had significantly higher tiller numbers and shorter plant heights. This study indicates that OsPAP10c encodes a novel secreted APase that plays an important role in the utilization of external organic P in rice.
dc.description.statementofresponsibilityLinghong Lu, Wenmin Qiu, Wenwen Gao, Stephen D. Tyerman, Huixia Shou, Chuang Wang
dc.identifier.citationPlant, Cell and Environment, 2016; 39(10):2247-2259
dc.identifier.doi10.1111/pce.12794
dc.identifier.issn0140-7791
dc.identifier.issn1365-3040
dc.identifier.orcidTyerman, S. [0000-0003-2455-1643]
dc.identifier.urihttp://hdl.handle.net/2440/103813
dc.language.isoen
dc.publisherWiley-Blackwell
dc.relation.granthttp://purl.org/au-research/grants/arc/CE140100008
dc.rights© 2016 John Wiley & Sons Ltd
dc.source.urihttps://doi.org/10.1111/pce.12794
dc.subjectOryza sativa (rice)
dc.subjectpurple acid phosphatase (PAP)
dc.titleOsPAP10c, a novel secreted acid phosphatase in rice, plays an important role in the utilization of external organic phosphorus
dc.typeJournal article
pubs.publication-statusPublished

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