Establishing human lacrimal gland cultures from biopsy-sized tissue specimens

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dc.contributor.authorHalliday, L.A.
dc.contributor.authorWood, J.P.M.
dc.contributor.authorChidlow, G.
dc.contributor.authorCasson, R.J.
dc.contributor.authorSelva, D.
dc.contributor.authorSun, M.T.
dc.date.issued2023
dc.description.abstractOBJECTIVES: To establish cultures of human lacrimal gland from patient-derived, biopsy-sized, tissue specimens. METHODS: Tissue was obtained after surgical removal from patients without dry eye disease undergoing routine procedures. Samples were subjected to mechanical and enzymatic digestion and resulting cell suspensions were plated onto collagen-coated glass coverslips and grown for up to 21 days. Cultures were analysed by immunocytochemistry and light microscopy, and resultant cellular distributions were compared to those in sections of fixed human lacrimal gland tissue. RESULTS: Dissociation of biopsy-sized pieces of human lacrimal gland and seeding onto coated surfaces allowed development of a mixed population of cells in vitro. Within 7–14 days, cellular aggregation was observed and by 21 days many cells had organised themselves into distinct three-dimensional complexes. Immunohistochemistry revealed a heterogeneous population of cells, including epithelial, myoepithelial, mesenchymal and progenitor cells. Some of the epithelia labelled positively for lysozyme and lactoferrin. CONCLUSIONS: Collection and dissociation of biopsy-sized pieces of human lacrimal gland leads to a cellular preparation that can proliferate in vitro and organise into three-dimensional structures. This is the first report detailing that biopsy-collected specimens of human lacrimal gland can be used to establish cell cultures.
dc.description.statementofresponsibilityLuke A. Halliday, John P. M. Wood, Glyn Chidlow, Robert J. Casson, Dinesh Selva and Michelle T. Sun
dc.identifier.citationEye, 2023; 37(1):62-68
dc.identifier.doi10.1038/s41433-021-01872-9
dc.identifier.issn0950-222X
dc.identifier.issn1476-5454
dc.identifier.orcidHalliday, L.A. [0000-0002-2253-0761]
dc.identifier.orcidChidlow, G. [0000-0001-7371-0239]
dc.identifier.orcidCasson, R.J. [0000-0003-2822-4076]
dc.identifier.orcidSelva, D. [0000-0002-2169-5417]
dc.identifier.orcidSun, M.T. [0000-0002-8066-9278]
dc.identifier.urihttps://hdl.handle.net/2440/138104
dc.language.isoen
dc.publisherSpringer
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/1183278
dc.rights© Crown 2021
dc.source.urihttps://doi.org/10.1038/s41433-021-01872-9
dc.subjectImmunohistochemistry
dc.subject.meshLacrimal Apparatus
dc.subject.meshCells, Cultured
dc.subject.meshEpithelial Cells
dc.subject.meshHumans
dc.subject.meshBiopsy
dc.subject.meshImmunohistochemistry
dc.titleEstablishing human lacrimal gland cultures from biopsy-sized tissue specimens
dc.typeJournal article
pubs.publication-statusPublished

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