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Versican accumulation in human prostatic fibroblast cultures is enhanced by prostate cancer cell-derived transforming growth factor beta1

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dc.contributor.authorSakko, A.
dc.contributor.authorRicciardelli, C.
dc.contributor.authorMayne, K.
dc.contributor.authorTilley, W.
dc.contributor.authorLeBaron, R.
dc.contributor.authorHorsfall, D.
dc.date.issued2001
dc.description© 2000 American Association for Cancer Research
dc.description.abstractWe have previously demonstrated that peritumoral stromal matrix derived from prostate cancer patients who relapse after radical surgery contains elevated levels of versican. The purpose of this study was to determine whether prostate cancer cells control stromal cell secretion of versican. Serum-free conditioned medium from three prostate adenocarcinoma cell lines, LNCaP, PC3, and DU145, was added to cultures of fibroblasts established from prostatic tissue of patients with benign prostatic hyperplasia, and the medium was harvested at 24, 48, and 72 h. Immunoblotting with an antiversican core protein antibody revealed that prostatic fibroblast medium harvested at 72 h contained increased levels of versican after treatment with either LNCaP-, PC3- or DU145-conditioned medium (2.5-, 4.5-, and 5-fold, respectively) compared with control cultures. This increase in versican in the culture medium was not observed after coincubation with transforming growth factor ß1-neutralizing antisera. The results of this study suggest that prostate tumor cells induce host stromal cells to secrete increased versican levels via a paracrine mechanism mediated by transforming growth factor ß1.
dc.description.statementofresponsibilityAndrew J. Sakko, Carmela Ricciardelli, Keiko Mayne, Wayne D. Tilley, Richard G. LeBaron, and David J. Horsfall
dc.identifier.citationCancer Research, 2001; 61(3):926-930
dc.identifier.issn0008-5472
dc.identifier.issn1538-7445
dc.identifier.orcidRicciardelli, C. [0000-0001-7415-1854]
dc.identifier.orcidTilley, W. [0000-0003-1893-2626]
dc.identifier.urihttp://hdl.handle.net/2440/34691
dc.language.isoen
dc.publisherAmer Assoc Cancer Research
dc.source.urihttp://cancerres.aacrjournals.org/cgi/content/abstract/61/3/926
dc.subjectProstate
dc.subjectCells, Cultured
dc.subjectFibroblasts
dc.subjectStromal Cells
dc.subjectHumans
dc.subjectAdenocarcinoma
dc.subjectProstatic Neoplasms
dc.subjectTransforming Growth Factor beta
dc.subjectLectins, C-Type
dc.subjectRNA, Messenger
dc.subjectCulture Media, Conditioned
dc.subjectImmunoblotting
dc.subjectCoculture Techniques
dc.subjectReverse Transcriptase Polymerase Chain Reaction
dc.subjectCell Communication
dc.subjectCell Division
dc.subjectMale
dc.subjectTransforming Growth Factor beta1
dc.subjectVersicans
dc.subjectChondroitin Sulfate Proteoglycans
dc.titleVersican accumulation in human prostatic fibroblast cultures is enhanced by prostate cancer cell-derived transforming growth factor beta1
dc.typeJournal article
pubs.publication-statusPublished

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