Metabolic flux analysis of hydrogen production network by Clostridium butyricum W5: Effect of pH and glucose concentrations

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dc.contributor.authorCai, G.
dc.contributor.authorJin, B.
dc.contributor.authorSaint, C.
dc.contributor.authorMonis, P.
dc.date.issued2010
dc.description.abstractFermentative hydrogen production by strict anaerobes has been widely reported. There is a lack of information related to metabolic flux distribution and its variation with respect to fermentation conditions in the metabolic production system. This study aimed to get a better understanding of the metabolic network and to conduct metabolic flux analysis (MFA) of fermentative hydrogen production by a recently isolated Clostridium butyricum strain W5. We chose the specific growth rate as the objective function and used specific H<inf>2</inf> production rate as the criterion to evaluate the experimental results with the in silico MFA. For the first time, we constructed an in silico metabolic flux model for the anaerobic glucose metabolism of C. butyricum W5 with assistance of a modeling program MetaFluxNet. The model was used to evaluate metabolic flux distribution in the fermentative hydrogen production network, and to study the fractional flux response to variations in initial glucose concentration and operational pH. The MFA results suggested that pH has a more significant effect on hydrogen production yield compared to the glucose concentration. The MFA is a useful tool to provide valuable information for optimization and design of the fermentative hydrogen production process. © 2010 Professor T. Nejat Veziroglu. Published by Elsevier Ltd. All rights reserved.
dc.description.statementofresponsibilityGuiqin Cai, Bo Jin, Chris Saint, Paul Monis
dc.identifier.citationInternational Journal of Hydrogen Energy, 2010; 35(13):6681-6690
dc.identifier.doi10.1016/j.ijhydene.2010.04.097
dc.identifier.issn0360-3199
dc.identifier.issn1879-3487
dc.identifier.urihttp://hdl.handle.net/2440/61486
dc.language.isoen
dc.publisherPergamon-Elsevier Science Ltd
dc.rightsCopyright © 2010 Professor T. Nejat Veziroglu All rights reserved.
dc.source.urihttps://doi.org/10.1016/j.ijhydene.2010.04.097
dc.subjectMetabolic flux analysis
dc.subjectFermentative hydrogen production
dc.subjectClostridium butyricum
dc.subjectOperational pH
dc.subjectInitial glucose concentrations
dc.titleMetabolic flux analysis of hydrogen production network by Clostridium butyricum W5: Effect of pH and glucose concentrations
dc.typeJournal article
pubs.publication-statusPublished

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