Airway dysbiosis: Haemophilus influenza and Tropheryma in poorly controlled asthma

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dc.contributor.authorSimpson, J.
dc.contributor.authorDaly, J.
dc.contributor.authorBaines, K.
dc.contributor.authorYang, I.
dc.contributor.authorUpham, J.
dc.contributor.authorReynolds, P.
dc.contributor.authorHodge, S.
dc.contributor.authorJames, A.
dc.contributor.authorHugenholtz, P.
dc.contributor.authorWillner, D.
dc.contributor.authorGibson, P.
dc.date.issued2016
dc.description.abstractAsthma is a chronic inflammatory disorder of the airways where bacteria may act as protagonists of chronic inflammation. Little is known about the relation of airway inflammation to the presence of specific bacterial taxa. We sought to describe the sputum microbiome in adults with poorly controlled asthma. DNA was extracted from induced sputum and microbial communities were profiled using 16S rRNA pyrosequencing. Bacterial species were characterised, and the relationship between microbial populations, asthma inflammatory subtypes and other covariates was explored. Real-time PCR was used to identify Tropheryma whipplei and Haemophilus influenzae in sputum. Adults with neutrophilic asthma had reduced bacterial diversity and species richness. Tropheryma was identified and confirmed with real-time PCR in 12 (40%) participants. Haemophilus occurred most often in a group of younger atopic males with an increased proportion of neutrophils. PCR confirmed the presence of H. influenzae in 35 (76%) participants with poorly controlled asthma. There are phenotype-specific alterations to the airway microbiome in asthma. Reduced bacterial diversity combined with a high prevalence of H. influenzae was observed in neutrophilic asthma, whereas eosinophilic asthma had abundant T. whipplei.
dc.description.statementofresponsibilityJodie L. Simpson, Joshua Daly, Katherine J. Baines, Ian A. Yang, John W. Upham, Paul N. Reynolds, Sandra Hodge, Alan L. James, Philip Hugenholtz, Dana Willner and Peter G. Gibson
dc.identifier.citationEuropean Respiratory Journal, 2016; 47(3):792-800
dc.identifier.doi10.1183/13993003.00405-2015
dc.identifier.issn0903-1936
dc.identifier.issn1399-3003
dc.identifier.orcidReynolds, P. [0000-0002-2273-1774]
dc.identifier.orcidHodge, S. [0000-0002-3602-9927] [0000-0002-9401-298X]
dc.identifier.urihttp://hdl.handle.net/2440/102789
dc.language.isoen
dc.publisherEuropean Respiratory Society
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/569246
dc.rightsCopyright ©ERS 2016
dc.source.urihttps://doi.org/10.1183/13993003.00405-2015
dc.subjectRespiratory System
dc.subjectNeutrophils
dc.subjectSputum
dc.subjectHumans
dc.subjectHaemophilus influenzae
dc.subjectAsthma
dc.subjectPulmonary Eosinophilia
dc.subjectInflammation
dc.subjectRNA, Ribosomal, 16S
dc.subjectLinear Models
dc.subjectAdult
dc.subjectAged
dc.subjectMiddle Aged
dc.subjectFemale
dc.subjectMale
dc.subjectTropheryma
dc.subjectMicrobiota
dc.subjectDysbiosis
dc.titleAirway dysbiosis: Haemophilus influenza and Tropheryma in poorly controlled asthma
dc.typeJournal article
pubs.publication-statusPublished

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