Serum-free culture of wool follicles: effects of nutrients, growth factors and hormones

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dc.contributor.authorBates, E.
dc.contributor.authorHynd, P.
dc.contributor.authorPenno, N.
dc.contributor.authorNancarrow, M.
dc.date.issued1997
dc.description.abstractA serum-free culture system allowed the continued growth of fibre from wool follicles for 8–10 days. Fibre growth was responsive to changes in the level of calcium, glucose and amino acids in the culture medium, and was stimulated by the inclusion of insulin (10μg/mL) in the medium. Culture of follicles in the presence of conditioned media from dermal papilla cells or of mitomycin-treated dermal papilla cellsl had no effect on fibre growth. Neither thyroid hormones nor hydrocortisone altered fibre growth. The progressive decline in fibre growth during follicle culture was accompanied by morphological changes in the follicle bulb. Oxidative damage did not appear to be the cause of these changes as there was no increase in fibre growth rate or longevity when antioxidants were used. This model provides a useful system to study the direct effects of various hormonal, nutritional and growth factors on fibre growth and follicle metabolism.
dc.identifier.citationBritish Journal of Dermatology, 1997; 137(4):498-505
dc.identifier.doi10.1111/j.1365-2133.1997.tb03778.x
dc.identifier.issn0007-0963
dc.identifier.issn1365-2133
dc.identifier.orcidHynd, P. [0000-0001-6123-8037]
dc.identifier.urihttp://hdl.handle.net/2440/13217
dc.language.isoen
dc.publisherOXFORD UNIV PRESS
dc.source.urihttps://doi.org/10.1111/j.1365-2133.1997.tb03778.x
dc.subjectHair Follicle
dc.subjectWool
dc.subjectSkin
dc.subjectAnimals
dc.subjectSheep
dc.subjectCalcium
dc.subjectReactive Oxygen Species
dc.subjectHydrocortisone
dc.subjectHormones
dc.subjectThyroid Hormones
dc.subjectGrowth Substances
dc.subjectMicronutrients
dc.subjectCulture Media, Serum-Free
dc.subjectCulture Techniques
dc.titleSerum-free culture of wool follicles: effects of nutrients, growth factors and hormones
dc.typeJournal article
pubs.publication-statusPublished

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