Characterisation of six α-expansin genes in Gossypium hirsutum (upland cotton)

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dc.contributor.authorHarmer, S.
dc.contributor.authorOrford, S.
dc.contributor.authorTimmis, J.
dc.date.issued2002
dc.descriptionThe original publication can be found at www.springerlink.com
dc.description.abstractA genomic library screen and PCR-based strategies were employed to isolate six genes with sequence similarity to a cotton fibre-specific mRNA encoding an α-expansin. α-Expansins are cell wall proteins that facilitate cell wall extension by disruption of non-covalent bonds between wall components. The characterisation and expression analysis of these six novel genes (GhExp1-GhExp6) is described. Four of them (GhExp3-GhExp6) are expressed within multiple tissues of the cotton plant and two (GhExp1 and GhExp2) give rise to transcripts that are specific to the developing cotton fibre. GhExp1 transcripts are highly abundant in the fibre, while transcripts for GhExp2 are detected at a low level. Cotton fibres are highly elongated cells of the ovule epidermis, and we envisage that GhExp1 may play an important role in cell wall extension during development.
dc.description.statementofresponsibilityS.E. Harmer, S.J. Orford, J.N. Timmis
dc.identifier.citationMolecular Genetics and Genomics, 2002; 268(1):1-9
dc.identifier.doi10.1007/s00438-002-0721-2
dc.identifier.issn1617-4615
dc.identifier.issn1617-4623
dc.identifier.urihttp://hdl.handle.net/2440/28138
dc.language.isoen
dc.publisherSpringer-Verlag
dc.rights© 2002 Springer-Verlag
dc.source.urihttps://doi.org/10.1007/s00438-002-0721-2
dc.subjectExpansin
dc.subjectGossypium hirsutum
dc.subjectCotton fibre
dc.subjectRT-PCR
dc.subjectGene family
dc.titleCharacterisation of six α-expansin genes in Gossypium hirsutum (upland cotton)
dc.title.alternativeCharacterisation of six alpha-expansin genes in Gossypium hirsutum (upland cotton)
dc.typeJournal article
pubs.publication-statusPublished

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