Rapid and selective detection of TP53 mutations in cancer using a novel conductometric biosensor
Date
2025
Authors
Perera, G.S.
Huang, X.
Bagherjeri, F.A.
Joglekar, C.M.
Leo, P.
Duijf, P.
Bhaskaran, M.
Sriram, S.
Punyadeera, C.
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Journal article
Citation
Biosensors and Bioelectronics, 2025; 276:1-8
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Abstract
Tumour protein p53 (TP53) is a tumour suppressor gene that is frequently mutated in cancers. Traditional TP53 detection methods, such as polymerase chain reactions, are time-consuming and demand skilled laboratory personnel. As an alternative, in the current study, we have demonstrated a high resistivity silicon (HR-Si) based conductometric biosensor designed for the rapid and specific identification of TP53 point mutations directly at the point-of-need. This biosensor accurately detected R248Q and R248W point mutant single strand DNA (ssDNA) as models, in real-time. Both R248Q and R248W mutant ssDNA exhibited a limit of detection (LOD) of 0.5 ng/mL in human plasma. The selectivity studies revealed that both R248Q and R248W mutant ssDNA can be detected 10 × lower molar content against their wild-type ssDNA. Validation of the sensor using clinical samples harbouring known TP53 mutations demonstrated a sensitivity of 100%, a specificity of 100%, and a LOD of 2.5 ng/mL. This precision biosensing platform at the point-of-need has the potential to revolutionise cancer diagnostics.
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Data source: Supplementary data, https://doi.org/10.1016/j.bios.2025.117252
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Copyright 2025 The Author(s). Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/)