A RhoGEF and Rho family GTPase-activating protein complex links the contractile ring to cortical microtubules at the onset of cytokinesis

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dc.contributor.authorSomers, W.
dc.contributor.authorSaint, R.
dc.contributor.organisationCentre for the Molecular Genetics of Development
dc.date.issued2003
dc.description.abstractThe mechanism that positions the cytokinetic contractile ring is unknown, but derives from the spindle midzone. We show that an interaction between the Rho GTP exchange factor, Pebble, and the Rho family GTPase-activating protein, RacGAP50C, connects the contractile ring to cortical microtubules at the site of furrowing in D. melanogaster cells. Pebble regulates actomyosin organization, while RacGAP50C and its binding partner, the Pavarotti kinesin-like protein, regulate microtubule bundling. All three factors are required for cytokinesis. As furrowing begins, these proteins colocalize to a cortical equatorial ring. We propose that RacGAP50C-Pavarotti complexes travel on cortical microtubules to the cell equator, where they associate with the Pebble RhoGEF to position contractile ring formation and coordinate F-actin and microtubule remodeling during cytokinesis.
dc.identifier.citationDevelopmental Cell, 2003; 4(1):29-39
dc.identifier.doi10.1016/S1534-5807(02)00402-1
dc.identifier.issn1534-5807
dc.identifier.issn1878-1551
dc.identifier.urihttp://hdl.handle.net/2440/2970
dc.language.isoen
dc.publisherCell Press
dc.source.urihttps://doi.org/10.1016/s1534-5807(02)00402-1
dc.subjectMicrotubules
dc.subjectAnimals
dc.subjectDrosophila melanogaster
dc.subjectMacromolecular Substances
dc.subjectGTPase-Activating Proteins
dc.subjectGuanine Nucleotide Exchange Factors
dc.subjectMicrotubule-Associated Proteins
dc.subjectDrosophila Proteins
dc.subjectRNA, Messenger
dc.subjectTwo-Hybrid System Techniques
dc.subjectCell Division
dc.subjectProtein Binding
dc.subjectRho Guanine Nucleotide Exchange Factors
dc.subjectSpindle Apparatus
dc.subjectWings, Animal
dc.titleA RhoGEF and Rho family GTPase-activating protein complex links the contractile ring to cortical microtubules at the onset of cytokinesis
dc.typeJournal article
pubs.publication-statusPublished

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