Matrix-assisted laser desorption/ionization imaging protocol for in situ characterization of tryptic peptide identity and distribution in formalin-fixed tissue

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dc.contributor.authorGustafsson, O.
dc.contributor.authorEddes, J.
dc.contributor.authorMeding, S.
dc.contributor.authorMcColl, S.
dc.contributor.authorOehler, M.
dc.contributor.authorHoffmann, P.
dc.date.issued2013
dc.description.abstract<h4>Rationale</h4>Matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry provides the means to map the in situ distribution of tryptic peptides in formalin-fixed clinical tissue samples. The ability to analyze clinical samples is of great importance to further developments in the imaging field. However, there is a requirement in this field of research for additional methods describing the characterization of tryptic peptides by MALDI imaging.<h4>Methods and results</h4>This protocol gives highly detailed instructions, with examples, for (1) successfully performing tryptic peptide MALDI imaging on formalin-fixed cancer tissue using a MALDI-TOF/TOF MS instrument, (2) tentatively generating identifications through nLC/MS/MS, and (3) validating these identifications by in situ MS/MS of peptides of interest.<h4>Conclusions</h4>This protocol provides a detailed and straightforward description of the methods required for groups new to MALDI imaging to begin analysis of formalin-fixed clinical samples.
dc.description.statementofresponsibilityOve J. R. Gustafsson, James S. Eddes, Stephan Meding, Shaun R. McColl, Martin K. Oehler and Peter Hoffmann
dc.identifier.citationRapid Communications in Mass Spectrometry, 2013; 27(6):655-670
dc.identifier.doi10.1002/rcm.6488
dc.identifier.issn0951-4198
dc.identifier.issn1097-0231
dc.identifier.orcidMcColl, S. [0000-0003-0949-4660]
dc.identifier.orcidOehler, M. [0000-0002-2651-5913]
dc.identifier.urihttp://hdl.handle.net/2440/78386
dc.language.isoen
dc.publisherJohn Wiley & Sons Ltd
dc.relation.granthttp://purl.org/au-research/grants/arc/LP110100693
dc.relation.granthttp://purl.org/au-research/grants/arc/LP110100693
dc.rightsCopyright © 2013 John Wiley & Sons, Ltd.
dc.source.urihttps://doi.org/10.1002/rcm.6488
dc.subjectHumans
dc.subjectNeoplasms
dc.subjectFormaldehyde
dc.subjectTrypsin
dc.subjectPeptide Fragments
dc.subjectCalibration
dc.subjectSpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
dc.subjectHistocytological Preparation Techniques
dc.subjectAmino Acid Sequence
dc.subjectImage Processing, Computer-Assisted
dc.subjectMolecular Sequence Data
dc.subjectTandem Mass Spectrometry
dc.subjectMolecular Imaging
dc.titleMatrix-assisted laser desorption/ionization imaging protocol for in situ characterization of tryptic peptide identity and distribution in formalin-fixed tissue
dc.typeJournal article
pubs.publication-statusPublished

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