3D bioprinting of a cell-laden antibacterial polysaccharide hydrogel composite
Date
2021
Authors
Rastin, H.
Ramezanpour, M.
Hassan, K.
Mazinani, A.
Tung, T.T.
Vreugde, S.
Losic, D.
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Journal article
Citation
Carbohydrate Polymers, 2021; 264:117989-1-117989-12
Statement of Responsibility
Hadi Rastin, Mahnaz Ramezanpour, Kamrul Hassan, Arash Mazinani, Tran Thanh Tung, Sarah Vreugde, Dusan Losic
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Abstract
Bioink with inherent antibacterial activity is of particular interest for tissue engineering application due to the growing number of bacterial infections associated with impaired wound healing or bone implants. However, the development of cell-laden bioink with potent antibacterial activity while supporting tissue regeneration proved to be challenging. Here, we introduced a cell-laden antibacterial bioink based on Methylcellulose/Alginate (MC/ Alg) hydrogel for skin tissue engineering via elimination of the risks associated with a bacterial infection. The key feature of the bioink is the use of gallium (Ga+3) in the design of bioink formulation with dual functions. First, Ga+3 stabilized the hydrogel bioink by the formation of ionic crosslinking with Alg chains. Second, the gallium- crosslinked bioink exhibited potent antibacterial activity toward both Gram-positive (Staphylococcus aureus) and Gram-negative (Pseudomonas aeruginosa) bacteria with a bactericidal rate of 99.99 %. In addition, it was found that the developed bioink supported encapsulated fibroblast cellular functions.
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Crown Copyright © 2021 Published by Elsevier Ltd. All rights reserved.