The role of N-terminal heterocycles in hydrogen bonding to α-chymotrypsin

Date

2019

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Schumann, N.C.
Bruning, J.
Marshall, A.C.
Abell, A.D.

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Bioorganic and Medicinal Chemistry Letters, 2019; 29(3):396-399

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Nicholas C.Schumann, John Bruning, Andrew C.Marshall, Andrew D.Abell

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Abstract

A series of dipeptide aldehydes containing different N-terminal heterocycles was prepared and assayed in vitro against α-chymotrypsin to ascertain the importance of the heterocycle in maintaining a β-strand geometry while also providing a hydrogen bond donor equivalent to the backbone amide nitrogen of the surrogate amino acid. The dipeptide containing a pyrrole constraint (10) was the most potent inhibitor, with >30-fold improved activity over dipeptides which lacked a nitrogen hydrogen bond donor (namely thiophene 11, furan 12 and pyridine 13). Molecular docking studies of 10 bound to α-chymotrypsin demonstrates a hydrogen bond between the pyrrole nitrogen donor and the backbone carbonyl of Gly216 located in the S3 pocket which is proposed to be critical for overall binding.

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© 2018 Elsevier Ltd. All rights reserved.

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