Genomic analysis of Kazachstania aerobia and Kazachstania servazzii reveals duplication of genes related to acetate ester production

Simple item page
dc.contributor.authorLin, M.M.H.
dc.contributor.authorWalker, M.E.
dc.contributor.authorJiranek, V.
dc.contributor.authorSumby, K.M.
dc.date.issued2023
dc.description.abstractKazachstania aerobia and Kazachstania servazzii can affect wine aroma by increasing acetate ester concentrations, most remarkably phenylethyl acetate and isoamyl acetate. The genetic basis of this is unknown, there being little to no sequence data available on the genome architecture. We report for the first time the near-complete genome sequence of the two species using long-read (PacBio) sequencing (K. aerobia 20 contigs, one scaffold; and K. servazzii 22 contigs, one scaffold). The annotated genomes of K. aerobia (12.5Mb) and K. servazzii (12.3Mb) were compared to Saccharomyces cerevisiae genomes (laboratory strain S288C and wine strain EC1118). Whilst a comparison of the two Kazachstania spp. genomes revealed few differences between them, divergence was evident in relation to the genes involved in ester biosynthesis, for which gene duplications or absences were apparent. The annotations of these genomes are valuable resources for future research into the evolutionary biology of Kazachstania and other yeast species (comparative genomics) as well as understanding the metabolic processes associated with alcoholic fermentation and the production of secondary ‘aromatic’ metabolites (transcriptomics, proteomics and metabolomics).
dc.description.statementofresponsibilityMandy Man-Hsi Lin, Michelle E. Walker, Vladimir Jiranek, and Krista M. Sumby
dc.identifier.citationMicrobial genomics, 2023; 9(6):1-14
dc.identifier.doi10.1099/mgen.0.001029
dc.identifier.issn2057-5858
dc.identifier.issn2057-5858
dc.identifier.orcidLin, M.M.H. [0000-0001-6781-1068]
dc.identifier.orcidWalker, M.E. [0000-0002-6934-3787]
dc.identifier.orcidJiranek, V. [0000-0002-9775-8963]
dc.identifier.orcidSumby, K.M. [0000-0002-1833-2025]
dc.identifier.urihttps://hdl.handle.net/2440/138759
dc.language.isoen
dc.publisherMicrobiology Society
dc.relation.granthttp://purl.org/au-research/grants/arc/IC70100008
dc.rights© 2023 The Authors. This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
dc.source.urihttps://doi.org/10.1099/mgen.0.001029
dc.subjectPacBio; whole genome sequencing; Kazachstania aerobia; Kazachstania servazzii; ester genes; wine aroma
dc.subject.meshSaccharomycetales
dc.subject.meshSaccharomyces cerevisiae
dc.subject.meshAcetates
dc.subject.meshGenomics
dc.titleGenomic analysis of Kazachstania aerobia and Kazachstania servazzii reveals duplication of genes related to acetate ester production
dc.typeJournal article
pubs.publication-statusPublished

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
hdl_138759.pdf
Size:
1.34 MB
Format:
Adobe Portable Document Format
Description:
Published version
Download

Collections

Agriculture, Food and Wine publications