Surface engineering of porous silicon to optimise therapeutic antibody loading and release

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dc.contributor.authorMcInnes, S.
dc.contributor.authorTurner, C.
dc.contributor.authorAl-Bataineh, S.
dc.contributor.authorAiraghi Leccardi, M.
dc.contributor.authorIrani, Y.
dc.contributor.authorWilliams, K.
dc.contributor.authorCowin, A.
dc.contributor.authorVoelcker, N.
dc.date.issued2015
dc.description.abstractThe proinflammatory cytokine, tumor necrosis factor-α (TNF-α), is elevated in several diseases such as uveitis, rheumatoid arthritis and non-healing chronic wounds. Adding Infliximab, a chimeric IgG1 monoclonal antibody raised against TNF-α, to chronic wound fluid can neutralise human TNF-α, thereby providing a potential therapeutic option for chronic wound healing. However, to avoid the need for repeated application in a clinical setting, and to protect the therapeutic antibody from the hostile environment of the wound, suitable delivery vehicles are required. Porous silicon (pSi) is a biodegradable high surface area material commonly employed for drug delivery applications. In this study, the use of pSi microparticles (pSi MPs) for the controlled release of Infliximab to disease environments, such as chronic wounds, is demonstrated. Surface chemistry and pore parameters for Infliximab loading are first optimised in pSi films and loading conditions are transferred to pSi MPs. Loading regimens exceeding 60 μg of Infliximab per mg of pSi are achieved. Infliximab is released with zero-order release kinetics over the course of 8 days. Critically, the released antibody remains functional and is able to sequester TNF-α over a weeklong timeframe; suitable for a clinical application in chronic wound therapy.
dc.description.statementofresponsibilitySteven J.P. McInnes, Chris T. Turner, Sameer A. Al-Bataineh, Marta J.I. Airaghi Leccardi, Yazad Irani, Keryn A. Williams, Allison J. Cowin and Nicolas H. Voelcker
dc.identifier.citationJournal of Materials Chemistry B, 2015; 3(20):4123-4133
dc.identifier.doi10.1039/c5tb00397k
dc.identifier.issn2050-750X
dc.identifier.issn2050-7518
dc.identifier.orcidIrani, Y. [0000-0002-2861-5774]
dc.identifier.orcidCowin, A. [0000-0003-2885-2080]
dc.identifier.urihttp://hdl.handle.net/2440/109910
dc.language.isoen
dc.publisherRoyal Society of Chemistry
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/595901
dc.relation.granthttp://purl.org/au-research/grants/arc/CE140100036
dc.rightsThis journal is © The Royal Society of Chemistry 2015
dc.source.urihttps://doi.org/10.1039/c5tb00397k
dc.titleSurface engineering of porous silicon to optimise therapeutic antibody loading and release
dc.typeJournal article
pubs.publication-statusPublished

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