The role of zinc efflux during Acinetobacter baumannii infection

Date

2020

Authors

Alquethamy, S.
Adams, F.G.
Naidu, V.
Khorvash, M.
Pederick, V.G.
Zang, M.
Paton, J.C.
Paulsen, I.T.
Hassan, K.
Cain, A.K.

Editors

Advisors

Journal Title

Journal ISSN

Volume Title

Type:

Journal article

Citation

ACS Infectious Diseases, 2020; 6(1):150-158

Statement of Responsibility

Saleh F. Alquethamy, Felise G. Adams, Varsha Naidu, Marjan Khorvash, Victoria G. Pederick, Maoge Zang, James C. Paton, Ian T. Paulsen, Karl A. Hassan, Amy K. Cain, Christopher A. McDevitt, and Bart A. Eijkelkamp

Conference Name

DOI

10.1021/acsinfecdis.9b00351

Abstract

Acinetobacter baumannii is a highly ubiquitous Gram-negative bacterium, that is associated with significant disease in immunocompromised individuals. The success of A. baumannii is partly attributable to its high level of antibiotic resistance. Further, A. baumannii expresses a broad arsenal of putative zinc efflux systems that are likely to aid environmental persistence and host colonisation, but detailed insights into how the bacterium deals with toxic concentrations of zinc is lacking. In this study we present the transcriptomic responses of A. baumannii to toxic zinc concentrations. Subsequent mutant analyses revealed a primary role for the resistance-nodulation-cell division heavy metal efflux system CzcCBA, and the cation diffusion facilitator transporter CzcD in zinc resistance. To examine the role of zinc at the host-pathogen interface we utilised a murine model of zinc deficiency and challenge with wild-type and czcA mutant cells, which identified highly site-specific roles for zinc during A. baumannii infection. Overall, we provide novel insight into the key zinc resistance mechanisms of A. baumannii and outline the role these systems play in enabling the bacterium to survive in diverse environments.

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Dissertation Note

Provenance

Description

Access Status

Rights

© 2019 American Chemical Society

License

Grant ID

http://purl.org/au-research/grants/nhmrc/1159752
 http://purl.org/au-research/grants/nhmrc/1080784
 http://purl.org/au-research/grants/nhmrc/1122582
 http://purl.org/au-research/grants/nhmrc/1071659
 http://purl.org/au-research/grants/arc/DP170102102
 http://purl.org/au-research/grants/nhmrc/1120298
 http://purl.org/au-research/grants/arc/FT170100006

Published Version

https://doi.org/10.1021/acsinfecdis.9b00351

Call number

Persistent link to this record

http://hdl.handle.net/2440/124680