Current studentsExisting staffContact us
 

Infrared spectrum analysis of the dissociated states of simple amino acids

Simple item page
dc.contributor.authorSebben, D.A.
dc.contributor.authorPendleton, P.
dc.date.issued2014
dc.description.abstractIn this work, we present detailed analyses of the dissociation of dilute aqueous solutions of glycine and of lysine over the range 1 < pH < 12. Using appropriate spectrum subtraction methods, we obtained ATR-IR spectra of the solvated species as a function of pH. Discernible changes in the ionic species were identified in the absorption region between 1800 and 1100 cm -1. By applying peak deconvolution techniques to the spectra, we correctly interpret the apparent peak shift from 1615 to 1600 cm 1 as being due to the receding NH+3 asymmetric deformation alongside the appearing COO- asymmetric stretching. The effect of aqueous solution environment was also investigated in terms of 10 and 100 mmol/L NaCl. Salt solution spectra at each pH were also subtracted from each solution phase spectrum. Analysis of the deconvoluted peak areas due to C=O and COO at pH ranges < 4.5 and those due to NH2 and NH+3 for pH > 8 resulted in consistent pKa values for the amino acids.
dc.identifier.citationSpectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, 2014; 132:706-712
dc.identifier.doi10.1016/j.saa.2014.05.026
dc.identifier.issn1386-1425
dc.identifier.issn1873-3557
dc.identifier.orcidPendleton, P. [0000-0003-1031-8170]
dc.identifier.urihttps://hdl.handle.net/1959.8/158703
dc.language.isoen
dc.publisherElsevier
dc.relation.fundingUniversity of South Australia
dc.rightsCopyright 2014 Elsevier BV
dc.source.urihttps://doi.org/10.1016/j.saa.2014.05.026
dc.subjectamino acids
dc.subjectATR-IR
dc.subjectglycine
dc.subjectlysine
dc.subjectsolution spectroscopy
dc.subjectSodium Chloride
dc.subjectIons
dc.subjectSolutions
dc.subjectSolvents
dc.subjectSpectroscopy, Fourier Transform Infrared
dc.subjectHydrogen-Ion Concentration
dc.subjectOsmolar Concentration
dc.titleInfrared spectrum analysis of the dissociated states of simple amino acids
dc.typeJournal article
pubs.publication-statusPublished
ror.mmsid9915910153901831

Files

Collections