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Ionized gas (plasma) delivery of reactive oxygen species (ROS) into artificial cells

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dc.contributor.authorHong, S.H.
dc.contributor.authorSzili, E.J.
dc.contributor.authorJenkins, A.T.A.
dc.contributor.authorShort, R.D.
dc.date.issued2014
dc.descriptionData source: Supplementary data, https://stacks.iop.org/JPhysD/47/362001/mmedia Link to a related website: https://doi.org/10.1088/0022-3727/48/2/029501, Corrigendum
dc.description.abstractThis study was designed to enhance our understanding of how reactive oxygen species (ROS), generated ex situ by ionized gas (plasma), can affect the regulation of signalling processes within cells. A model system, comprising of a suspension of phospholipid vesicles (cell mimics) encapsulating a ROS reporter, was developed to study the plasma delivery of ROS into cells. For the first time it was shown that plasma unequivocally delivers ROS into cells over a sustained period and without compromising cell membrane integrity. An important consideration in cell and biological assays is the presence of serum, which significantly reduced the transfer efficiency of ROS into the vesicles. These results are key to understanding how plasma treatments can be tailored for specific medical or biotechnology applications. Further, the phospholipid vesicle ROS reporter system may find use in other studies involving the application of free radicals in biology and medicine.
dc.identifier.citationJournal Physics D: Applied Physics, 2014; 47(36, article no. 362001):1-6
dc.identifier.doi10.1088/0022-3727/47/36/362001
dc.identifier.issn0022-3727
dc.identifier.issn1361-6463
dc.identifier.urihttps://hdl.handle.net/1959.8/160204
dc.language.isoen
dc.publisherInstitute of Physics Publishing Ltd
dc.relation.fundingWound Management Innovation CRC , Project: RP 2.11
dc.relation.fundingBacteriosafe KOALA 295155
dc.rightsCopyright 2014 IOP Publishing
dc.source.urihttps://doi.org/10.1088/0022-3727/47/36/362001
dc.subject7-dichlorodihydrofluorescein (DCFH)
dc.subjectartificial cells
dc.subjectfree radicals
dc.subjectphospholipid vesicles
dc.subjectplasma jet
dc.subjectplasma medicine
dc.subjectreactive oxygen species (ROS)
dc.titleIonized gas (plasma) delivery of reactive oxygen species (ROS) into artificial cells
dc.typeJournal article
pubs.publication-statusPublished
ror.mmsid9915910132301831

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