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Quantitative proteomics reveals that plasma membrane microdomains from poplar cell suspension cultures are enriched in markers of signal transduction, molecular transport, and callose biosynthesis

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dc.contributor.authorSrivastava, V.
dc.contributor.authorMalm, E.
dc.contributor.authorSundqvis, G.
dc.contributor.authorBulone, V.
dc.date.issued2013
dc.description.abstractThe plasma membrane (PM) is a highly dynamic interface that contains detergent-resistant microdomains (DRMs). The aim of this work was to determine the main functions of such microdomains in poplar through a proteomic analysis using gel-based and solution (iTRAQ) approaches. A total of 80 proteins from a limited number of functional classes were found to be significantly enriched in DRM relative to PM. The enriched proteins are markers of signal transduction, molecular transport at the PM, or cell wall biosynthesis. Their intrinsic properties are presented and discussed together with the biological significance of their enrichment in DRM. Of particular importance is the significant and specific enrichment of several callose [(1 → 3)-β-glucan] synthase isoforms, whose catalytic activity represents a final response to stress, leading to the deposition of callose plugs at the surface of the PM. An integrated functional model that connects all DRM-enriched proteins identified is proposed. This report is the only quantitative analysis available to date of the protein composition of membrane microdomains from a tree species.
dc.description.statementofresponsibilityVaibhav Srivastava, Erik Malm, Gustav Sundqvist, and Vincent Bulone
dc.identifier.citationMolecular & Cellular Proteomics, 2013; 12(12):3874-3885
dc.identifier.doi10.1074/mcp.M113.029033
dc.identifier.issn1535-9476
dc.identifier.issn1535-9484
dc.identifier.orcidBulone, V. [0000-0002-9742-4701] [0000-0003-2809-4160]
dc.identifier.urihttp://hdl.handle.net/2440/90315
dc.language.isoen
dc.publisherAmerican Society for Biochemistry and Molecular Biology
dc.rights© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.
dc.source.urihttps://doi.org/10.1074/mcp.m113.029033
dc.subjectMembrane Microdomains
dc.subjectCell Wall
dc.subjectPopulus
dc.subjectGlucans
dc.subjectIsoenzymes
dc.subjectGlucosyltransferases
dc.subjectCell Culture Techniques
dc.subjectProteomics
dc.subjectSignal Transduction
dc.subjectGene Expression Regulation, Plant
dc.subjectBiological Transport
dc.subjectMass Spectrometry
dc.subjectStress, Physiological
dc.subjectMolecular Sequence Annotation
dc.subjectPlant Cells
dc.titleQuantitative proteomics reveals that plasma membrane microdomains from poplar cell suspension cultures are enriched in markers of signal transduction, molecular transport, and callose biosynthesis
dc.typeJournal article
pubs.publication-statusPublished

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