A microbioreactor based on interfacial polymerisation and application to flow injection analysis of glucose
Date
1996
Authors
Son, M.
Peddie, F.
Mulcahy, D.
Davey, D.
Haskard, M.R.
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Journal article
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Sensors and Actuators, B: Chemical, 1996; 34(1-3):422-428
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Abstract
A miniature bioreactor is described that uses micromachining fabrication techniques. The bioreactor chamber is created by anisotropic etching of a silicon wafer and attached to a microelectronic sensor by epoxy resin. Any biocatalyst such as an enzyme, a combination of enzymes, or intact viable cells can be contained within the microscopic chamber by an ultra-thin nylon membrane. Tight sealing of the membrane to the silicon wafer surface is achieved by pretreating the anisotropically etched wafer surface with a silylating reagent and then creating the membrane by interfacial polymerisation. By appropriate selection of aqueous and organic phase constituents, the nylon membrane can be covalently linked to the wafer surface by amide bonds. As a simple demonstration for the usefulness of this concept, the microbioreactor has been configured as a glucose sensor in a flow injection cell. Initial results are encouraging and demonstrate that many other applications are feasible.
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Copyright 1996 Elsevier Science S.A.