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Improving the safety of viral DNA vaccines: development of vectors containing both 5' and 3' homologous regulatory sequences from non-viral origin

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dc.contributor.authorMartinez Lopez, A.
dc.contributor.authorEncinas, P.
dc.contributor.authorGarcía Valtanen, P.
dc.contributor.authorGomez Casado, E.
dc.contributor.authorColl, J.M.
dc.contributor.authorEstepa, A.
dc.date.issued2013
dc.description.abstractAlthough some DNA vaccines have proved to be very efficient in field trials, their authorisation still remains limited to a few countries. This is in part due to safety issues because most of them contain viral regulatory sequences to driving the expression of the encoded antigen. This is the case of the only DNA vaccine against a fish rhabdovirus (a negative ssRNA virus), authorised in Canada, despite the important economic losses that these viruses cause to aquaculture all over the world. In an attempt to solve this problem and using as a model a non-authorised, but efficient DNA vaccine against the fish rhabdovirus, viral haemorrhagic septicaemia virus (VHSV), we developed a plasmid construction containing regulatory sequences exclusively from fish origin. The result was an "all-fish vector", named pJAC-G, containing 5′ and 3′ regulatory sequences of β-acting genes from carp and zebrafish, respectively. In vitro and in vivo, pJAC-G drove a successful expression of the VHSV glycoprotein G (G), the only antigen of the virus conferring in vivo protection. Furthermore, and by means of in vitro fusion assays, it was confirmed that G protein expressed from pJAC-G was fully functional. Altogether, these results suggest that DNA vaccines containing host-homologous gene regulatory sequences might be useful for developing safer DNA vaccines, while they also might be useful for basic studies.
dc.identifier.citationApplied Microbiology and Biotechnology, 2013; 97(7):3007-3016
dc.identifier.doi10.1007/s00253-012-4403-7
dc.identifier.issn0175-7598
dc.identifier.issn1432-0614
dc.identifier.urihttps://hdl.handle.net/11541.2/115057
dc.language.isoen
dc.publisherSpringer
dc.relation.fundingMICINN AGL2011-28921-C03-01 and -02
dc.relation.fundingMICINN CONSOLIDER INGENIO 2010-CSD2007-00002
dc.relation.fundingGeneralitat Valenciana ISIC-2012-003
dc.rightsCopyright 2012 Springer-Verlag
dc.source.urihttps://doi.org/10.1007/s00253-012-4403-7
dc.subjectDNA
dc.subjectfish
dc.subjectplasmid
dc.subjectrhabdovirus
dc.subjectterminators
dc.subjectvaccines
dc.subjectvector regulatory sequences
dc.subjectVHSV
dc.titleImproving the safety of viral DNA vaccines: development of vectors containing both 5' and 3' homologous regulatory sequences from non-viral origin
dc.typeJournal article
pubs.publication-statusPublished
ror.mmsid9915933159101831

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