Cloning and characterization of the 5'-flanking region of the human transcription factor Sp1 gene
Date
2001
Authors
Nicolas, M.
Noe, V.
Jensen, K.
Ciudad, C.
Editors
Advisors
Journal Title
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Volume Title
Type:
Journal article
Citation
Journal of Biological Chemistry, 2001; 276(25):22126-22132
Statement of Responsibility
Marta Nicolás, Vèronique Noé, Kirk B. Jensen, and Carlos J. Ciudad
Conference Name
Abstract
The 5'-flanking region of the human Sp1 gene was cloned and characterized. Sequence analysis of this region showed the absence of both CAAT and TATA boxes and an initiator element. The proximal promoter of the Sp1 gene is a GC-rich region that contains multiple GC boxes and Ap2 binding sites. The major transcription start site is located 63 base pairs upstream of the translation start site. Transfection experiments demonstrate that all the elements necessary to achieve significant basal transcription activity are located between positions 443 and 20 relative to the translational start. Sp1 and Sp3 proteins bind to the downstream GC box located in the proximal promoter of Sp1. Furthermore, we demonstrate that the Sp1 protein activates Sp1 transcription activity; thus the Sp1 gene is autoregulated.