Biosynthesis, characterisation and direct high-performance liquid chromatographic analysis of gemfibrozil 1-O-β-acylglucuronide

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dc.contributor.authorSallustio, B.
dc.contributor.authorFairchild, B.
dc.date.issued1995
dc.description.abstractGemfibrozil 1-O-β-acylglucuronide was purified from the urine of a volunteer administered gemfibrozil, and an isocratic reversed-phase HPLC method was developed for its direct measurement. Quantitation of gemfibrozil and gemfibrozil 1-O-β-acylglucuronide was carried out from plasma, following extraction from acidified specimens into ethyl acetate, on a 5-μm CN reversed-phase column with a mobile phase (pH 3.5) containing acetonitrile, tetrabutylammonium sulphate and distilled water, using fluorescence detection at 284 nm excitation and 316 nm emission. Calibration curves were linear for both compounds over a concentration range of 0.1 to 40 mg/l, with intra-assay coefficients of variation <5% at concentrations of 20.0, 2.0 and 0.2 mg/l, and inter-assay coefficients of variation <10%. No degradation of gemfibrozil 1-O-β-acylglucuronide was detected as a result of the analytical procedure. However, a preliminary application of the method indicates that gemfibrozil acylglucuronide is chemically unstable undergoing intra-molecular rearrangement and hydrolysis under physiological conditions.
dc.description.statementofresponsibilityBenedetta C. Sallustio, Barbara A. Fairchild
dc.identifier.citationJournal of Chromatography A, 1995; 665(2):345-353
dc.identifier.doi10.1016/0378-4347(94)00530-I
dc.identifier.issn0021-9673
dc.identifier.issn1879-1794
dc.identifier.orcidSallustio, B. [0000-0002-0186-3073]
dc.identifier.urihttp://hdl.handle.net/2440/14460
dc.language.isoen
dc.publisherElsevier
dc.rightsCopyright © 1995 Published by Elsevier B.V.
dc.source.urihttps://doi.org/10.1016/0378-4347(94)00530-i
dc.subjectHumans
dc.subjectGemfibrozil
dc.subjectGlucuronates
dc.subjectChromatography, High Pressure Liquid
dc.subjectChromatography, Thin Layer
dc.subjectMagnetic Resonance Spectroscopy
dc.subjectDrug Stability
dc.subjectHydrogen-Ion Concentration
dc.subjectQuality Control
dc.subjectMass Spectrometry
dc.titleBiosynthesis, characterisation and direct high-performance liquid chromatographic analysis of gemfibrozil 1-O-β-acylglucuronide
dc.title.alternativeBiosynthesis, characterisation and direct high-performance liquid chromatographic analysis of gemfibrozil 1-O-beta-acylglucuronide
dc.typeJournal article
pubs.publication-statusPublished

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