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Evaluation of heterotrophic plate and chromogenic agar colony counting in water quality laboratories

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dc.contributor.authorHallas, G.
dc.contributor.authorMonis, P.
dc.date.issued2015
dc.description.abstractThe enumeration of bacteria using plate-based counts is a core technique used by food and water microbiology testing laboratories. However, manual counting of bacterial colonies is both time and labour intensive, can vary between operators and also requires manual entry of results into laboratory information management systems, which can be a source of data entry error. An alternative is to use automated digital colony counters, but there is a lack of peer-reviewed validation data to allow incorporation into standards. We compared the performance of digital counting technology (ProtoCOL3) against manual counting using criteria defined in internationally recognized standard methods. Digital colony counting provided a robust, standardized system suitable for adoption in a commercial testing environment. The digital technology has several advantages:Improved measurement of uncertainty by using a standard and consistent counting methodology with less operator error.Efficiency for labour and time (reduced cost).Elimination of manual entry of data onto LIMS.Faster result reporting to customers.
dc.identifier.citationMethodsX, 2015; 2:415-422
dc.identifier.doi10.1016/j.mex.2015.10.003
dc.identifier.issn2215-0161
dc.identifier.issn2215-0161
dc.identifier.urihttps://hdl.handle.net/11541.2/141083
dc.language.isoen
dc.publisherElsevier
dc.rightsCopyright 2015 Elsevier B.V. This is an open access article under the CC BY license. (https://creativecommons.org/licenses/by/4.0/)
dc.source.urihttps://doi.org/10.1016/j.mex.2015.10.003
dc.subjectdigital
dc.subjectheterotrophic plate count
dc.subjectwater
dc.subjectcfu
dc.subjectmembrane filtration
dc.subjectchromogenic agar
dc.titleEvaluation of heterotrophic plate and chromogenic agar colony counting in water quality laboratories
dc.typeJournal article
pubs.publication-statusPublished
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ror.mmsid9916331706601831

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