Current studentsExisting staffContact us
 

Development of a robust protocol for gene expression analysis using formalin-fixed, paraffin-embedded liver transplant biopsy specimens

Simple item page
dc.contributor.authorThompson, E.
dc.contributor.authorBurt, A.
dc.contributor.authorBarker, C.
dc.contributor.authorKirby, J.
dc.contributor.authorBrain, J.
dc.date.issued2013
dc.description.abstractLiver transplant biopsies are routinely archived following formalin fixation and paraffin embedding and may provide an additional source of diagnostic information following transcriptomic biomarker analysis. This study was designed to compare gene transcription between resting and stressed biliary cells in culture, these cells after fixation and embedding and archival liver transplant biopsy tissue. The transcription of p21/WAF1 and transforming growth factor (TGF)-β1 showed similar changes in the fresh and embedded liver cells. However, the expression of TGF-β2 was markedly different between the fresh and embedded samples, suggesting that fixation can produce sequence-specific artefacts. Sufficient quantities of pure RNA were recovered from all the liver transplant biopsies to allow complementary DNA production. Measurement of the transcription of all three genes showed variability between the cases. Although the results for individual transcripts should be interpreted with care, these data do suggest the feasibility of performing a larger biomarker discovery studies using archival tissue.
dc.description.statementofresponsibilityEmily Thompson, Alastair D Burt, Catriona E Barker, John A Kirby, John G Brain
dc.identifier.citationJournal of Clinical Pathology, 2013; 66(9):815-818
dc.identifier.doi10.1136/jclinpath-2013-201548
dc.identifier.issn0021-9746
dc.identifier.issn1366-8714
dc.identifier.orcidBurt, A. [0000-0002-3011-7774]
dc.identifier.urihttp://hdl.handle.net/2440/87057
dc.language.isoen
dc.publisherBMJ Publishing Group
dc.rightsThis is an Open Access article distributed in accordance with the terms of the Creative Commons Attribution (CC BY 3.0) license, which permits others to distribute, remix, adapt and build upon this work, for commercial use, provided the original work is properly cited. See: http://creativecommons.org/licenses/by/3.0/
dc.source.urihttps://doi.org/10.1136/jclinpath-2013-201548
dc.subjectLiver
dc.subjectHumans
dc.subjectFormaldehyde
dc.subjectLiver Transplantation
dc.subjectParaffin Embedding
dc.subjectTissue Fixation
dc.subjectGene Expression Profiling
dc.subjectGene Expression
dc.titleDevelopment of a robust protocol for gene expression analysis using formalin-fixed, paraffin-embedded liver transplant biopsy specimens
dc.typeJournal article
pubs.publication-statusPublished

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
hdl_87057.pdf
Size:
378.44 KB
Format:
Adobe Portable Document Format
Description:
Published version
Download

Collections

Medicine publications