Construction of an ~700-kb transcript map around the Familial Mediterranean Fever locus on human chromosome 16p13.3

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dc.contributor.authorCentola, M.
dc.contributor.authorChen, X.
dc.contributor.authorSood, R.
dc.contributor.authorDeng, Z.
dc.contributor.authorAksentijevich, I.
dc.contributor.authorBlake, T.
dc.contributor.authorRicke, D.
dc.contributor.authorChen, X.
dc.contributor.authorWood, G.
dc.contributor.authorZaks, N.
dc.contributor.authorRichards, N.
dc.contributor.authorKrizman, D.
dc.contributor.authorMansfield, E.
dc.contributor.authorApostolou, S.
dc.contributor.authorLiu, J.
dc.contributor.authorShafran, N.
dc.contributor.authorVedula, A.
dc.contributor.authorHamon, M.
dc.contributor.authorCercek, A.
dc.contributor.authorKahan, T.
dc.contributor.authoret al.
dc.date.issued1998
dc.description.abstractWe used a combination of cDNA selection, exon amplification, and computational prediction from genomic sequence to isolate transcribed sequences from genomic DNA surrounding the familial Mediterranean fever (FMF) locus. Eighty-seven kb of genomic DNA around D16S3370, a marker showing a high degree of linkage disequilibrium with FMF, was sequenced to completion, and the sequence annotated. A transcript map reflecting the minimal number of genes encoded within the ∼700 kb of genomic DNA surrounding the FMF locus was assembled. This map consists of 27 genes with discreet messages detectable on Northerns, in addition to three olfactory-receptor genes, a cluster of 18 tRNA genes, and two putative transcriptional units that have typical intron–exon splice junctions yet do not detect messages on Northerns. Four of the transcripts are identical to genes described previously, seven have been independently identified by the French FMF Consortium, and the others are novel. Six related zinc-finger genes, a cluster of tRNAs, and three olfactory receptors account for the majority of transcribed sequences isolated from a 315-kb FMF central region (betweenD16S468/D16S3070 and cosmid 377A12). Interspersed among them are several genes that may be important in inflammation. This transcript map not only has permitted the identification of the FMF gene (MEFV), but also has provided us an opportunity to probe the structural and functional features of this region of chromosome 16.
dc.description.statementofresponsibilityMichael Centola, Xiaoguang Chen, Raman Sood, Zuoming Deng, Ivona Aksentijevich, Trevor Blake, Darrell O. Ricke, Xiang Chen, Geryl Wood, Nurit Zaks, Neil Richards, David Krizman, Elizabeth Mansfield, Sinoula Apostolou, Jingmei Liu, Neta Shafran, Anil Vedula, Melanie Hamon, Andrea Cercek, Tanaz Kahan, Deborah Gumucio, David F. Callen, Robert I. Richards, Robert K. Moyzis, Norman A. Doggett, Francis S. Collins, P. Paul Liu, Nathan Fischel-Ghodsian and Daniel L. Kastner
dc.identifier.citationGenome Research, 1998; 8(11):1172-1191
dc.identifier.doi10.1101/gr.8.11.1172
dc.identifier.issn1088-9051
dc.identifier.issn1549-5469
dc.identifier.orcidCallen, D. [0000-0002-6189-9991]
dc.identifier.urihttp://hdl.handle.net/2440/11437
dc.language.isoen
dc.publisherCOLD SPRING HARBOR LAB PRESS
dc.rightsCopyright © 1998 by Cold Spring Harbor Laboratory Press
dc.source.urihttp://genome.cshlp.org/cgi/content/abstract/8/11/1172
dc.subjectChromosomes, Human, Pair 16
dc.subjectHumans
dc.subjectFamilial Mediterranean Fever
dc.subjectReceptors, Odorant
dc.subjectDNA
dc.subjectDNA, Complementary
dc.subjectRNA, Transfer
dc.subjectPhysical Chromosome Mapping
dc.subjectCloning, Molecular
dc.subjectSequence Analysis, DNA
dc.subjectTranscription, Genetic
dc.subjectGene Amplification
dc.subjectAmino Acid Sequence
dc.subjectZinc Fingers
dc.subjectSequence Homology, Amino Acid
dc.subjectGenes
dc.subjectMultigene Family
dc.subjectGenome, Human
dc.subjectExons
dc.subjectMolecular Sequence Data
dc.titleConstruction of an ~700-kb transcript map around the Familial Mediterranean Fever locus on human chromosome 16p13.3
dc.typeJournal article
pubs.publication-statusPublished

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