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Induction of a grapevine germin-like protein (VvGLP3) gene is closely linked to the site of Erysiphe necator infection: A possible role in defense?

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dc.contributor.authorGodfrey, D.
dc.contributor.authorAble, A.
dc.contributor.authorDry, I.
dc.date.issued2007
dc.descriptionCopyright © 2007 The American Phytopathological Society
dc.description.abstractGermin-like proteins (GLP) have various proposed roles in plant development and defense. Seven novel GLP cDNA clones were isolated from grapevine (Vitis vinifera cv. Chardonnay). Reverse transcriptase-polymerase chain reaction expression analysis revealed that the VvGLP genes exhibit diverse and highly specific patterns of expression in response to a variety of abiotic and biotic treatments, including challenge by Erysiphe necator, Plasmopara viticola, and Botrytis cinerea, suggesting a diversity of roles for each of the GLP family members. Significantly, one of the grapevine GLP genes, VvGLP3, is induced specifically by E. necator infection and expression is closely linked to the site of infection. Subcellular localization of VvGLP3 determined by transient expression of a VvGLP3:GFP fusion construct in onion cells indicated that the recombinant protein was targeted to the cell wall. Recombinant VvGLP3 was successfully expressed in Arabidopsis thaliana and the partially purified recombinant protein was demonstrated to have superoxide dismutase activity. This data has provided an insight into the diverse nature of the GLP family in grapevine and suggests that VvGLP3 may be involved in the defense response against E. necator.
dc.identifier.citationMolecular Plant-Microbe Interactions, 2007; 20(9):1112-1125
dc.identifier.doi10.1094/MPMI-20-9-1112
dc.identifier.issn0894-0282
dc.identifier.issn1943-7706
dc.identifier.orcidAble, A. [0000-0003-1135-6515]
dc.identifier.urihttp://hdl.handle.net/2440/44170
dc.language.isoen
dc.publisherAmer Phytopathological Soc
dc.source.urihttp://apsjournals.apsnet.org/doi/abs/10.1094/MPMI-20-9-1112
dc.subjectAscomycota
dc.subjectArabidopsis
dc.subjectOnions
dc.subjectVitis
dc.subjectPlant Leaves
dc.subjectSalicylic Acid
dc.subjectEthylenes
dc.subjectCyclopentanes
dc.subjectSuperoxide Dismutase
dc.subjectPlant Proteins
dc.subjectRecombinant Proteins
dc.subjectCloning, Molecular
dc.subjectPhylogeny
dc.subjectSignal Transduction
dc.subjectPlant Diseases
dc.subjectGene Expression Regulation, Plant
dc.subjectAmino Acid Sequence
dc.subjectMultigene Family
dc.subjectMolecular Sequence Data
dc.subjectOxylipins
dc.titleInduction of a grapevine germin-like protein (VvGLP3) gene is closely linked to the site of Erysiphe necator infection: A possible role in defense?
dc.typeJournal article
pubs.publication-statusPublished

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