Genetic and molecular characterization of the maize rp3 rust resistance locus

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dc.contributor.authorWebb, C.
dc.contributor.authorRichter, T.
dc.contributor.authorCollins, N.
dc.contributor.authorNicolas, M.
dc.contributor.authorTrick,, H.
dc.contributor.authorPryor, A.
dc.contributor.authorHulbert, S.
dc.date.issued2002
dc.description.abstractIn maize, the Rp3 gene confers resistance to common rust caused by Puccinia sorghi. Flanking marker analysis of rust-susceptible rp3 variants suggested that most of them arose via unequal crossing over, indicating that rp3 is a complex locus like rp1. The PIC13 probe identifies a nucleotide binding site-leucine-rich repeat (NBS-LRR) gene family that maps to the complex. Rp3 variants show losses of PIC13 family members relative to the resistant parents when probed with PIC13, indicating that the Rp3 gene is a member of this family. Gel blots and sequence analysis suggest that at least 9 family members are at the locus in most Rp3-carrying lines and that at least 5 of these are transcribed in the Rp3-A haplotype. The coding regions of 14 family members, isolated from three different Rp3-carrying haplotypes, had DNA sequence identities from 93 to 99%. Partial sequencing of clones of a BAC contig spanning the rp3 locus in the maize inbred line B73 identified five different PIC13 paralogues in a region of ~140 kb.
dc.description.statementofresponsibilityCraig A. Webb, Todd E. Richter, Nicholas C. Collins, Marie Nicolas, Harold N. Trick, Tony Pryor and Scot H. Hulbert
dc.identifier.citationGenetics: a periodical record of investigations bearing on heredity and variation, 2002; 162(1):381-394
dc.identifier.doi10.1093/genetics/162.1.381
dc.identifier.issn0016-6731
dc.identifier.issn1943-2631
dc.identifier.orcidCollins, N. [0000-0002-5447-6755]
dc.identifier.urihttp://hdl.handle.net/2440/34194
dc.language.isoen
dc.publisherGenetics
dc.rightsCopyright © 2002 by the Genetics Society of America
dc.source.urihttp://www.genetics.org/cgi/content/abstract/162/1/381
dc.subjectBasidiomycota
dc.subjectZea mays
dc.subjectPlant Proteins
dc.subjectDNA Primers
dc.subjectMeiosis
dc.subjectRecombination, Genetic
dc.subjectAmino Acid Sequence
dc.subjectBase Sequence
dc.subjectSequence Homology, Amino Acid
dc.subjectHaplotypes
dc.subjectPhenotype
dc.subjectGenes, Plant
dc.subjectMolecular Sequence Data
dc.titleGenetic and molecular characterization of the maize rp3 rust resistance locus
dc.typeJournal article
pubs.publication-statusPublished

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