Variation in ultrastructure of mucoid coat and shell membrane secretion of a dasyurid marsupial

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dc.contributor.authorRoberts, C.
dc.contributor.authorBreed, W.
dc.date.issued1996
dc.description.abstractIn the dasyurid marsupial Sminthopsis crassicaudata, the shell membrane of cleaving embryos has a compact granular structure but becomes fibrous around blastocysts. Polyclonal antibodies were raised against the extracellular coats, mucoid and shell membrane, of oocytes and early embryos. Immunogold cytochemistry resulted in labelling of secretory granules in the epithelia of both the ampulla and isthmus of the oviduct, although the secretory granules of these two regions differed in their ultrastructural appearance. Those in the ampulla were heterogeneous with areas of varying electron density, whereas those in the isthmus were electron dense and homogeneous. Shell membrane precursors were found in secretory granules in the epithelia of the uterotubal junction and endometrial glands and were electron lucent.
dc.description.statementofresponsibilityC. T. Roberts and W. G. Breed
dc.identifier.citationReproduction, Fertility and Development, 1996; 8(4):645-648
dc.identifier.doi10.1071/RD9960645
dc.identifier.issn1031-3613
dc.identifier.issn1448-5990
dc.identifier.orcidRoberts, C. [0000-0002-9250-2192]
dc.identifier.orcidBreed, W. [0000-0003-1739-6593]
dc.identifier.urihttp://hdl.handle.net/2440/5460
dc.language.isoen
dc.publisherCSIRO Publishing
dc.rights© CSIRO 1996
dc.source.urihttps://doi.org/10.1071/rd9960645
dc.subjectFallopian Tubes
dc.subjectUterus
dc.subjectZygote
dc.subjectMembranes
dc.subjectCytoplasmic Granules
dc.subjectMucus
dc.subjectGastrula
dc.subjectAnimals
dc.subjectMarsupialia
dc.subjectMicroscopy, Electron
dc.subjectImmunohistochemistry
dc.subjectEmbryonic and Fetal Development
dc.subjectFemale
dc.subjectEmbryo, Mammalian
dc.titleVariation in ultrastructure of mucoid coat and shell membrane secretion of a dasyurid marsupial
dc.typeJournal article
pubs.publication-statusPublished

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