Atomically flat gold for biomolecule immobilization and imaging
Date
2001
Authors
Losic, D.
Shapter, J.
Gooding, J.
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Journal article
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Australian Journal of Chemistry, 2001; 54(9-10):643-648
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Abstract
<jats:p>A method of easily fabricating gold surfaces that are atomically flat (less than 1 nm variation in height) over large areas using mica as a template is outlined. The smooth gold surfaces are prepared by vacuum-depositing gold onto mica surfaces maintained at 300°C. After deposition of the gold, the surfaces are annealed at 300°C for a further 16 h before removal from the vacuum chamber. Cryogenically stripping the gold from the mica revealed flat surfaces that allowed ‘defect-free’ self-assembled monolayers to be produced and the imaging of biomolecules with sufficient resolution to ascertain their orientation on the surface.</jats:p>
<jats:p>Manuscript received: 17 September 2001.</jats:p>
<jats:p>Revised version: 15 November 2001.</jats:p>
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