Efficacy of butyrate analogues in HT-29 cancer cells
Date
2010
Authors
Ooi, C.C.
Good, N.M.
Williams, D.B.
Lewanowitsch, T.
Cosgrove, L.J.
Lockett, T.J.
Head, R.J.
Editors
Advisors
Journal Title
Journal ISSN
Volume Title
Type:
Journal article
Citation
Clinical and Experimental Pharmacology and Physiology, 2010; 37(4):482-489
Statement of Responsibility
Conference Name
Abstract
Butyrate is a well known product of starch fermentation by colonic bacteria and is of interest owing to its ability to induce in vitro apoptosis and cell differentiation, as well as to inhibit cell growth in colorectal and other cancer cells. Synthetic analogues of butyrate may also possess cellular activities in a variety of cultured cells. The aim of the present study was to evaluate the effects of butyrate analogues on apoptosis, proliferation and histone deacetylase (HDAC) activity in HT-29 colorectal cancer cells. In addition, the effects of these analogues on lactate dehydrogenase leakage, as a measure of non-specific cytotoxicity, were evaluated in HT-29 cells.
In conclusion, chemical changes to the structure of butyrate can significantly modify the biological activity assayed in HT-29 colorectal cancer cells in vitro.
Of the 26 analogues examined, four (propionate, 4-benzoylbutyrate, 4-(4-aminophenyl)butyrate and benzyloxyacetate) exhibited comparable effects to butyrate. Interestingly, no activity was noted for compounds carrying amino, hydroxyl or methyl substitutions at the 2-, 3- or 4-position of the aliphatic moiety of butyrate.
School/Discipline
Dissertation Note
Provenance
Description
Data source: Supporting information, http://onlinelibrary.wiley.com/doi/10.1111/j.1440-1681.2009.05335.x/abstract
Access Status
Rights
Copyright 2010 The Authors journal compilation copyright 2010 Blackwell Publishing Asia Pty Ltd