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Imaging cells and sub-cellular structures with ultrahigh resolution full-field X-ray microscopy

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dc.contributor.authorChien, C.C.
dc.contributor.authorTseng, P.
dc.contributor.authorChen, H.
dc.contributor.authorHua, T.
dc.contributor.authorChen, S.T.
dc.contributor.authorChen, Y.
dc.contributor.authorLeng, W.H.
dc.contributor.authorWang, C.
dc.contributor.authorHwu, Y.
dc.contributor.authorYin, G.
dc.contributor.authorLiang, K.
dc.contributor.authorChen, F.
dc.contributor.authorChu, Y.
dc.contributor.authorYeh, H.
dc.contributor.authorYang, Y.
dc.contributor.authorYang, C.
dc.contributor.authorZhang, G.
dc.contributor.authorJe, J.
dc.contributor.authorMargaritondo, G.
dc.date.issued2013
dc.description.abstractOur experimental results demonstrate that full-field hard-X-ray microscopy is finally able to investigate the internal structure of cells in tissues. This result was made possible by three main factors: the use of a coherent (synchrotron) source of X-rays, the exploitation of contrast mechanisms based on the real part of the refractive index and the magnification provided by high-resolution Fresnel zone-plate objectives. We specifically obtained high-quality microradiographs of human and mouse cells with 29 nm Rayleigh spatial resolution and verified that tomographic reconstruction could be implemented with a final resolution level suitable for subcellular features. We also demonstrated that a phase retrieval method based on a wave propagation algorithm could yield good subcellular images starting from a series of defocused microradiographs. The concluding discussion compares cellular and subcellular hard-X-ray microradiology with other techniques and evaluates its potential impact on biomedical research.
dc.identifier.citationBiotechnology Advances, 2013; 31(3):375-386
dc.identifier.doi10.1016/j.biotechadv.2012.04.005
dc.identifier.issn0734-9750
dc.identifier.urihttps://hdl.handle.net/11541.2/115192
dc.language.isoen
dc.publisherElsevier
dc.source.urihttps://doi.org/10.1016/j.biotechadv.2012.04.005
dc.subjectfresnel phase zone plate
dc.subjectphase contrast radiology
dc.subjectsubcellular organelle
dc.subjectx-ray microscopy
dc.titleImaging cells and sub-cellular structures with ultrahigh resolution full-field X-ray microscopy
dc.typeJournal article
pubs.publication-statusPublished
ror.mmsid9915930099201831

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