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Item Metadata only Occurrence of anthocyanin pigments in berries of the white cultivar Muscat Gordo Blanco (Vitis vinifera L.)(Australian Society of Viticulture & Oenology, 1995) Gholami, Mansour; Coombe, B. G.Rose coloration of skin was observed to sometimes develop late during ripening on the normally white grape berries of cv. Muscat Gordo Blanco. The nature of the pigment was investigated by HPLC analysis of skin extracts of single berries. The predominant anthocyanin was identified as cyanidin-3-glucoside with minor amounts of delphinidin- and peonidin-3-glucosides. This composition resembles the skin composition of coloured, small-seeded muscat cultivars which it also resembles by the monoterpene composition of the juice. The pigments occurred only in berries with levels of total soluble solids in excess of 24°Brix in the juice and such berries tended to have smaller fresh weight. Berry pigmentation occurred on vines with various root systems. The specific conditions under which pigment developed in Muscat Gordo berries may offer a useful tool in the study of anthocyanin biosynthesis.Item Metadata only In vivo growth of a murine lymphoma cell line alters regulation of expression of Hsp72(American Society for Microbiology, 1995) Davidson, Sean; Hoj, Peter Bordier; Gabriele, Tim; Anderson, Robin L.We have identified a murine B-cell lymphoma cell line, CH1, that has a much-diminished capacity to express increased levels of heat shock proteins in response to heat stress in vitro. In particular, these cells cannot synthesize the inducible 72-kDa heat shock protein (HSP72) which is normally expressed at high levels in stressed cells. We show here that CH1 fails to transcribe HSP72 mRNA after heat shock, even though the heat shock transcription factor, HSF, is activated correctly. After heat shock, HSF from CH1 is found in the nucleus and is phosphorylated, trimerized, and capable of binding the heat shock element. We propose that additional signals which CH1 cells are unable to transduce are normally required to activate hsp72 transcription in vitro. Surprisingly, we have found that when the CH1 cells are heated in situ in a mouse, they show normal expression of HSP72 mRNA and protein. Therefore, CH1 cells have a functional hsp72 gene which can be transcribed and translated when the cells are in an appropriate environment. A diffusible factor present in ascites fluid is capable of restoring normal HSP72 induction in CH1 cells. We conclude that as-yet- undefined factors are required for regulation of the hsp72 gene or, alternatively, that heat shock in vivo causes activation of hsp70 through a novel pathway which the defect in CH1 has exposed and which is distinct from that operating in vitro. This unique system offers an opportunity to study a physiologically relevant pathway of heat shock induction and to biochemically define effectors involved in the mammalian stress response.Item Metadata only Phytophthora dieback in banksias: Screening for resistance(International Society for Horticultural Science, 1995) Tynan, K.; Scott, E.; Sedgley, M.; Dixon, K.; Sivasithamparam, K.Screening methods were investigated for assessing the resistance/susceptibility of Banksia species to Phytophthora infection. P. cinnamomi Rands was isolated most frequently from soil under infected Banksia plants in South Australia. Two isolates of P. citricola Sawada were also identified. Preliminary results indicated the response of excised shoot material to be quite variable and dependent on seasonal variations. The excised root assay showed potential as a routine screening assay. Micropropagation of B. coccinea has been successful and material will be included in an in vitro inoculation assay. Research continues into the handling of in vitro propagated plantlets.Item Metadata only Early bunchstem necrosis - A matter of nomenclature(American Society of Enologists, 1995) Jackson, D. I.; Coombe, B. G.Item Metadata only Contarinia bursariae, a new species of Cecidomyiidae (Diptera) infesting fruits of sweet bursaria, Bursaria spinosa (Pittosporaceae) in Australia(Royal Society of South Australia, 1995) Kolesik, PeterItem Metadata only A new species of Eocincticornia Felt (Diptera: Cecidomyiidae) on Eucalyptus fasciculosa in South Australia(Australian Entomological Society, 1995) Kolesik, PeterItem Metadata only Banksia coccinea 'Waite Crimson'(Australian Government Publishing Service, 1995) Sedgley, MargaretItem Metadata only Osmoregulation in water stressed roots: Responses of leaf conductance and photosynthesis(Institut fur Rebenzuchtung Geilweilerhof der Bundesanstalt fur Zuchtungsforschung an Kulturpflanzen, 1995) During, H.; Dry, P.Item Metadata only Biosynthesis of flavour compounds in Muscat Gordo Blanco grape berries(Australian Society of Viticulture & Oenology, 1995) Gholami, Mansour; Hayasaka, Yoji; Coombe, B. G.; Jackson, J. F.; Robinson, I.; Williams, P. J.Item Metadata only Fruit-set on small Chardonnay & Shiraz vines grown under varying temperature regimes between budburst and flowering(Australian Society of Viticulture & Oenology, 1995) Ebadi, Ali; Coombe, B. G.; May, P.Item Metadata only Reflections on the educational requirements of the grapegrowing and winemaking industry in the 21st century(Ryan Publications, 1995) Hoj, Peter BordierItem Metadata only Chemometric analysis of gas chromatographic data of oils from Eucalyptus species(Elsevier Science B.V., 1995) Dunlop, Peter J.; Bignell, Caroline M.; Jackson, J. F.; Hibbert, D. BrynnItem Metadata only A tedtrad of ionizable amino acids is important for catalysis in barley β-glucanases(The American Society for Biochemistry & Molecular Biology, 1995) Chen, Lin; Garrett, Thomas P. J.; Fincher, Geoffrey B.; Hoj, Peter BordierItem Metadata only Effect of pollen parent and stages of flower development on almond nut production(CSIRO, 1995) Vezvaei, Ali; Jackson, J. F.Item Metadata only Solution structure of the acetylated and noncleavable mitochondrial targeting signal of rat chaperonin 19(The American Society for Biochemistry & Molecular Biology, 1995) Jarvis, Jackie A.; Ryan, Michael T.; Hoogenraad, Nicholas J.; Craik, David J.; Hoj, Peter BordierItem Metadata only Arbitrarily primed PCR's diagnostic power(Thomson Publications, 1995) Liu, D.; Panaccio, M.; Van Heeswijck, Robyn; Latch, G.Item Metadata only A quarter century of mechanical grape harvesting in Australia(Winetitles, 1995) May, P.Item Metadata only Stereochemical course of glucan hydrolysis by barley (1 → 3)- and (1 → 3,1 → 4)-β-glucanases(Elsevier Science B.V., 1995) Chen, Lin; Sadek, Maruse; Stone, Bruce A.; Brownlee, Robert T. C.; Fincher, Geoffrey B.; Hoj, Peter BordierItem Metadata only Volatile Leaf Oils of some south-western and southern Australian species of the genus Eucalyptus. Part VI - subgenus symphyomyrtus, section adnataria(John Wiley & Sons Ltd., 1995) Bignell, Caroline M.; Dunlop, Peter J.; Brophy, J. J.; Jackson, J. F.Item Metadata only Crystal and molecular structure of g-L-glutamyl-b-cyano-L-alanine(Munksgaard, 1995) Delaere, I.; Tate, M.; Jones, G.; Tiekink, E.