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Type: Journal article
Title: Isolation and characterisation of a cDNA encoding a Zona Pellucida Protein (ZPB) from the marsupial Trichosurus vulpecula (Brushtail Possum)
Author: Haines, B.
Rathjen, P.
Hope, R.
Whyatt, L.
Holland, M.
Breed, W.
Citation: Molecular Reproduction and Development, 1999; 52(2):174-182
Publisher: WILEY
Issue Date: 1999
ISSN: 1040-452X
Abstract: We have cloned a cDNA containing the entire coding sequence of a marsupial (the brushtail possum, Trichosurus vulpecula) zona pellucida protein (ZPB). The open reading frame of 1,581 nt is predicted to encode a ZPB polypeptide of 527 amino acids which contains 20 cysteine residues, 7 potential N-linked glycosylation sites, a potential N-terminal signal peptide and a potential C-terminal trans-membrane domain, preceded by a furin proteolytic processing signal. Sequence comparisons between possum ZPB and orthologous polypeptides from 7 eutherian species and from Xenopus laevis, reveal the existence of a high degree of sequence similarity, particularly in the central portion of the molecule. Cysteine residues are highly conserved, and all nine species possess potential N-terminal signal peptide sequences and C-terminal trans-membrane domains of approximately the same length. In situ hybridisation revealed that expression of ZPB was restricted to oocytes of primordial and primary follicles of adult possums; no expression was detected in the surrounding granulosa cells. The broad conservation of ZPB sequence, structure and expression over a wide range of mammalian species, revealed by our studies, makes it unlikely that these features account for the different properties of the marsupial and eutherian zona pellucidae.
Keywords: Animals
Egg Proteins
Membrane Glycoproteins
Receptors, Cell Surface
DNA, Complementary
Gene Expression
Amino Acid Sequence
Base Sequence
Sequence Homology, Amino Acid
Molecular Sequence Data
Zona Pellucida Glycoproteins
Description: Article first published online: 4 JAN 1999
Rights: Copyright © 1999 Wiley-Liss, Inc.
DOI: 10.1002/(SICI)1098-2795(199902)52:2<174::AID-MRD8>3.0.CO;2-7
Appears in Collections:Aurora harvest 2
Environment Institute publications
Genetics publications

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