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Type: Journal article
Title: Globular structures in roots accumulate phosphorus to extremely high concentrations following phosphorus addition
Author: Ryan, M.H.
Kaur, P.
Nazeri, N.K.
Clode, P.L.
Keeble-Gagnère, G.
Doolette, A.L.
Smernik, R.J.
Van Aken, O.
Nicol, D.
Maruyama, H.
Ezawa, T.
Lambers, H.
Millar, A.H.
Appels, R.
Citation: Plant, Cell and Environment, 2019; 42(6):1987-2002
Publisher: Wiley Online Library
Issue Date: 2019
ISSN: 0140-7791
Statement of
Megan H. Ryan, Parwinder Kaur, Nazanin K. Nazeri, Peta L. Clode, Gabriel Keeble‐Gagnère
Abstract: Crops with improved uptake of fertilizer phosphorus (P) would reduce P losses and confer environmental benefits. We examined how P-sufficient 6-week-old soil-grown Trifolium subterraneum plants, and 2-week-old seedlings in solution culture, accumulated P in roots after inorganic P (Pi) addition. In contrast to our expectation that vacuoles would accumulate excess P, after 7 days, X-ray microanalysis showed that vacuolar [P] remained low (<12 mmol kg-1 ). However, in the plants after P addition, some cortex cells contained globular structures extraordinarily rich in P (often >3,000 mmol kg-1 ), potassium, magnesium, and sodium. Similar structures were evident in seedlings, both before and after P addition, with their [P] increasing threefold after P addition. Nuclear magnetic resonance (NMR) spectroscopy showed seedling roots accumulated Pi following P addition, and transmission electron microscopy (TEM) revealed large plastids. For seedlings, we demonstrated that roots differentially expressed genes after P addition using RNAseq mapped to the T. subterraneum reference genome assembly and transcriptome profiles. Among the most up-regulated genes after 4 hr was TSub_g9430.t1, which is similar to plastid envelope Pi transporters (PHT4;1, PHT4;4): expression of vacuolar Pi-transporter homologs did not change. We suggest that subcellular P accumulation in globular structures, which may include plastids, aids cytosolic Pi homeostasis under high-P availability.
Keywords: X-ray microanalysis
nuclear magnetic resonance spectroscopy
phosphorous efficient crops
Rights: © 2019 John Wiley & Sons Ltd.
DOI: 10.1111/pce.13531
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Appears in Collections:Agriculture, Food and Wine publications
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