Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/119408
Citations
Scopus Web of Science® Altmetric
?
?
Type: Journal article
Title: Globular structures in roots accumulate phosphorus to extremely high concentrations following phosphorus addition
Author: Ryan, M.
Kaur, P.
Nazeri, N.
Clode, P.
Keeble-Gagnère, G.
Doolette, A.
Smernik, R.
Van Aken, O.
Nicol, D.
Maruyama, H.
Ezawa, T.
Lambers, H.
Millar, A.
Appels, R.
Citation: Plant Cell and Environment, 2019; 42(6):1987-2002
Publisher: Wiley Online Library
Issue Date: 2019
ISSN: 0140-7791
1365-3040
Statement of
Responsibility: 
Megan H. Ryan, Parwinder Kaur, Nazanin K. Nazeri, Peta L. Clode, Gabriel Keeble‐Gagnère
Abstract: Crops with improved uptake of fertilizer phosphorus (P) would reduce P losses and confer environmental benefits. We examined how P-sufficient 6-week-old soil-grown Trifolium subterraneum plants, and 2-week-old seedlings in solution culture, accumulated P in roots after inorganic P (Pi) addition. In contrast to our expectation that vacuoles would accumulate excess P, after 7 days, X-ray microanalysis showed that vacuolar [P] remained low (<12 mmol kg-1 ). However, in the plants after P addition, some cortex cells contained globular structures extraordinarily rich in P (often >3,000 mmol kg-1 ), potassium, magnesium, and sodium. Similar structures were evident in seedlings, both before and after P addition, with their [P] increasing threefold after P addition. Nuclear magnetic resonance (NMR) spectroscopy showed seedling roots accumulated Pi following P addition, and transmission electron microscopy (TEM) revealed large plastids. For seedlings, we demonstrated that roots differentially expressed genes after P addition using RNAseq mapped to the T. subterraneum reference genome assembly and transcriptome profiles. Among the most up-regulated genes after 4 hr was TSub_g9430.t1, which is similar to plastid envelope Pi transporters (PHT4;1, PHT4;4): expression of vacuolar Pi-transporter homologs did not change. We suggest that subcellular P accumulation in globular structures, which may include plastids, aids cytosolic Pi homeostasis under high-P availability.
Keywords: X-ray microanalysis; nuclear magnetic resonance spectroscopy; phosphorous efficient crops; plastids; roots; vacuoles
Rights: © 2019 John Wiley & Sons Ltd.
RMID: 0030108265
DOI: 10.1111/pce.13531
Grant ID: http://purl.org/au-research/grants/arc/FT140100103
http://purl.org/au-research/grants/arc/CE140100008
Appears in Collections:Agriculture, Food and Wine publications

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.